Identification of new complex for caspase-2 activation after DNA damage
- Авторлар: Kopeina G.1, Zamaraev A.1, Zhivotovsky B.1,2, Lavrik I.1,3
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Мекемелер:
- Faculty of Basic Medicine
- Institute of Environmental Medicine
- Department of Translational Inflammation Research, Institute of Experimental Internal Medicine
- Шығарылым: Том 42, № 1 (2016)
- Беттер: 74-82
- Бөлім: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/227835
- DOI: https://doi.org/10.1134/S1068162016010064
- ID: 227835
Дәйексөз келтіру
Аннотация
Caspase-2 is reported to play an initiator role in apoptotic cell death in response to DNA damage. In this study, the mechanism of caspase-2 activation after DNA damage was investigated in human ovarian cancer cells Caov-4 treated with the chemotherapeutic agent cisplatin. To isolate the protein complex that might be involved in caspase-2 activation, a combination of gel filtration and immunoprecipitation was used. In the first step the high molecular weight complexes were separated from caspase-2 monomers by means of gel-filtration and in the second step immunoprecipitation from the high molecular weight gel-filtration fractions allowed us to isolate the complex that contains caspase-2. Interestingly, this complex did not contain the protein RAIDD that is a core component of the PIDDosome platform; the latter was shown to play an essential role in DNA damage-induced caspase-2 activation. Finally, catalytically active caspase-2 was detected in this complex, which indicates the possibility of formation of an alternative platform for caspase2 activation in DNA damage-induced apoptosis.
Негізгі сөздер
Авторлар туралы
G. Kopeina
Faculty of Basic Medicine
Email: ilav3103@gmail.com
Ресей, pr. Lomonosovsky 31/5, Moscow, 119991
A. Zamaraev
Faculty of Basic Medicine
Email: ilav3103@gmail.com
Ресей, pr. Lomonosovsky 31/5, Moscow, 119991
B. Zhivotovsky
Faculty of Basic Medicine; Institute of Environmental Medicine
Email: ilav3103@gmail.com
Ресей, pr. Lomonosovsky 31/5, Moscow, 119991; Box 210, Stockholm, 17177
I. Lavrik
Faculty of Basic Medicine; Department of Translational Inflammation Research, Institute of Experimental Internal Medicine
Хат алмасуға жауапты Автор.
Email: ilav3103@gmail.com
Ресей, pr. Lomonosovsky 31/5, Moscow, 119991; Magdeburg