Test-systems for immunoenzymatic determination of 6-deoxobrassinosteroids

R. P. Litvinovskaya; A. L. Sauchuk; K. G. Kazharnovich; A. G. Pradko; T. V. Mirantsova; V. N. Zhabinskii; V. A. Khripach

doi:10.1134/S1068162017030098

Test-systems for immunoenzymatic determination of 6-deoxobrassinosteroids

Authors: Litvinovskaya R.P.¹, Sauchuk A.L.¹, Kazharnovich K.G.¹, Pradko A.G.², Mirantsova T.V.², Zhabinskii V.N.¹, Khripach V.A.¹
Affiliations:
1. Institute of Bioorganic Chemistry
2. Hemma-Test Ltd.
Issue: Vol 43, No 3 (2017)
Pages: 286-301
Section: Article
URL: https://journals.rcsi.science/1068-1620/article/view/228524
DOI: https://doi.org/10.1134/S1068162017030098
ID: 228524

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Abstract

26-Hydroxyderivatives of 6-deoxo-24-epicastasterone and 6-deoxo-28-norcastasterone were synthesized for preparation of the corresponding haptens and conjugates with horseradish peroxidase and bovine serum albumin. These preparations were further applied to a production of antisera. Test-systems for the enzyme immunoassay of phytohormones of the 6-deoxobrassinosteroid group were elaborated.