Email this article Increase in Sensitivity of HEK293FT Cells to Influenza Infection by CRISPR-Cas9-Mediated Knockout of IRF7 Transcription Factor
- Authors: Komissarov A.B.1,2, Sergeeva M.V.1,2, Mozhaeva E.V.1, Eshchenko N.V.1, Vasilieva A.1,2, Vasilyev K.A.2, Medvedev S.P.1,3, Malakhova A.A.1,3, Balakhonova E.A.1, Malanin S.4, Grigoryeva T.V.4, Zhuravlev E.S.1, Semenov D.V.1, Richter V.1, Stepanov G.1
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Affiliations:
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
- Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation
- Federal Research Center Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
- Institute of Fundamental Medicine and Biology, Kazan Federal University
- Issue: Vol 45, No 6 (2019)
- Pages: 749-757
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229326
- DOI: https://doi.org/10.1134/S1068162019060232
- ID: 229326
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Abstract
Interferon-regulated factors play a central role in the activation of the innate immune response. The interferon-regulatory factor 7 (IRF7) is one of the factors that are quickly activated and are involved in a cellular response to a viral infection. In this work, monoclonal lines, based on HEK293FT cells defective in the IRF7 gene, were obtained using a CRISPR-Cas9 genome-editing method. These lines differed in the viability, proliferation rate, and susceptibility to infection with influenza A virus. Transcriptomic analysis of the most susceptible cell clone revealed differential expression of IRF7 factor as well as the other interferon-regulated genes.
Keywords
About the authors
A. B. Komissarov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences; Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090; St. Petersburg, 197376
M. V. Sergeeva
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences; Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090; St. Petersburg, 197376
E. V. Mozhaeva
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
N. V. Eshchenko
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
A. D. Vasilieva
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences; Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090; St. Petersburg, 197376
K. A. Vasilyev
Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation
Email: stepanovga@niboch.nsc.ru
Russian Federation, St. Petersburg, 197376
S. P. Medvedev
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences; Federal Research Center Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090; Novosibirsk, 630090
A. A. Malakhova
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences; Federal Research Center Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090; Novosibirsk, 630090
E. A. Balakhonova
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
S. Yu. Malanin
Institute of Fundamental Medicine and Biology, Kazan Federal University
Email: stepanovga@niboch.nsc.ru
Russian Federation, Kazan, 420008
T. V. Grigoryeva
Institute of Fundamental Medicine and Biology, Kazan Federal University
Email: stepanovga@niboch.nsc.ru
Russian Federation, Kazan, 420008
E. S. Zhuravlev
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
D. V. Semenov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
V. A. Richter
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
G. A. Stepanov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Author for correspondence.
Email: stepanovga@niboch.nsc.ru
Russian Federation, Novosibirsk, 630090
