Preliminary Structural Characteristic of Polysaccharides Extracted From the Callus Tissue of Sosnowskyi’s Hogweed (Heracleum Sosnowskyi Manden) Stem by Aqueous Ammonium Oxalate
- Authors: Gordina E.N.1, Kuznetsov S.P.2, Golovchenko V.V.3, Zlobin A.A.1
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Affiliations:
- Vyatka State University
- Institute of Chemistry, Komi Scientific Center, Ural Branch, Russian Academy of Sciences
- Institute of Physiology, Komi Scientific Center, Ural Branch, Russian Academy of Sciences
- Issue: Vol 45, No 6 (2019)
- Pages: 522-527
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229245
- DOI: https://doi.org/10.1134/S1068162019060165
- ID: 229245
Cite item
Abstract
Pectic polysaccharides have been isolated from callus tissue of the stem of Sosnowskyi’s hogweed (Heracleum sosnowskyi Manden) by extraction with a 0.7% aqueous solution of ammonium oxalate and characterized. It was shown that they differ from pectic polysaccharides of a native plant by a lower content of D‑galacturonic acid residues and a higher content of arabinose and galactose residues. The results obtained by a combination of ion exchange chromatography, enzymatic hydrolysis, periodate oxidation, methylation and NMR spectroscopy indicated the presence of low-methyl esterified 1,4-α-D-galacturonan, rhamogalacturonan-I, arabinan and galactan or arabinogalactan. The terminal 1,2- and 1,5-linked arabinofuranose residues, terminal, 1,3-, 1,6-linked and 3,6-substituted galactopyranose residues and 1,4-linked hexopyranose (galactopyranose or glucopyranose) residues were identified in the composition of neutral carbohydrate chains. The presence in the NMR spectra of trans-glycosyl correlation peaks of the methyl groups of 2,4-di-O-substituted rhamnose residues indirectly confirm the attachment arabinan, galactan or arabinogalactan carbohydrate chains to rhamnopyranose residues of the backbone at C-4 position.
About the authors
E. N. Gordina
Vyatka State University
Email: vgtu.aua@yandex.ru
Russian Federation, Kirov, 610000
S. P. Kuznetsov
Institute of Chemistry, Komi Scientific Center, Ural Branch, Russian Academy of Sciences
Email: vgtu.aua@yandex.ru
Russian Federation, Syktyvkar, 167982
V. V. Golovchenko
Institute of Physiology, Komi Scientific Center, Ural Branch, Russian Academy of Sciences
Email: vgtu.aua@yandex.ru
Russian Federation, Syktyvkar, 167982
A. A. Zlobin
Vyatka State University
Author for correspondence.
Email: vgtu.aua@yandex.ru
Russian Federation, Kirov, 610000