Email this article Specificity and Selectivity of a Modified Radioligand Method for Estimating the Binding Activity of β1 Adrenergic Receptors in Human T Lymphocytes
- Authors: Smolyakova E.V.1, Skoblov Y.S.2, Skoblova N.A.2, Agapova O.Y.3, Ambatiello L.G.1, Klimova A.A.1, Kuznetsova T.V.1, Masenko V.P.1, Nistor S.Y.3, Rvacheva A.V.3, Chazova I.E.1, Zykov K.A.4,3
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Affiliations:
- National Medical Research Center of Cardiology, Ministry of Healthcare of the Russian Federation
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Healthcare of the Russian Federation
- Research Institute of Pulmonology, Federal Medical and Biological Agency of Russia
- Issue: Vol 45, No 2 (2019)
- Pages: 129-134
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229174
- DOI: https://doi.org/10.1134/S1068162019020134
- ID: 229174
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Abstract
A radioligand-based method was proposed for quantifying the binding activity of β1-adrenergic receptors (ARs) on the surface of human T lymphocytes. Using the transfected HEK293 cell lines expressing β1-AR (ADL-7A) and β2-AR (A2R9) as a model, the conditions for [125I]cyanopindolol reliable detection of specific ligand binding to β1-adrenergic receptors at the level of 1–1.5 fmol per 1 million cells were selected. The real possibility of performing this analysis for clinical studies was shown by the example of human T lymphocytes.
About the authors
E. V. Smolyakova
National Medical Research Center of Cardiology, Ministry of Healthcare of the Russian Federation
Author for correspondence.
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 121552
Yu. S. Skoblov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 117997
N. A. Skoblova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 117997
O. Yu. Agapova
Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Healthcare of the Russian Federation
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 127473
L. G. Ambatiello
National Medical Research Center of Cardiology, Ministry of Healthcare of the Russian Federation
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 121552
A. A. Klimova
National Medical Research Center of Cardiology, Ministry of Healthcare of the Russian Federation
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 121552
T. V. Kuznetsova
National Medical Research Center of Cardiology, Ministry of Healthcare of the Russian Federation
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 121552
V. P. Masenko
National Medical Research Center of Cardiology, Ministry of Healthcare of the Russian Federation
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 121552
S. Yu. Nistor
Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Healthcare of the Russian Federation
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 127473
A. V. Rvacheva
Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Healthcare of the Russian Federation
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 127473
I. E. Chazova
National Medical Research Center of Cardiology, Ministry of Healthcare of the Russian Federation
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 121552
K. A. Zykov
Research Institute of Pulmonology, Federal Medical and Biological Agency of Russia; Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Healthcare of the Russian Federation
Email: smolyakovak@mail.ru
Russian Federation, Moscow, 115682; Moscow, 127473
