Effect of the B Subunit of the Cholera Toxin on the Raw 264.7 Murine Macrophage-Like Cell Line


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Аннотация

The 125I-labeled B-subunit of the cholera toxin ([125I]CT-B with specific activity 98 Ci/mmol) was found to be bonded to the murine macrophage-like cells of the RAW 264.7 line with high affinity (Kd 2.3 nM). The binding of the 125I-labeled CT-B was inhibited by the unlabeled interferon-α2 (IFN-α2), thymosin-α1, (TM-α1), and the LKEKK synthetic peptide corresponding to the 16–20 sequence of human TM-α1 and 131–135 sequence of human IFN-α2 (Ki 0.9, 1.1, and 1.4 nM, respectively), but the KKEKL unlabeled synthetic peptide with the inverted sequence did not inhibit binding (Ki > 1 μM). In the concentration range from 10 to 1000 nM, CT-B and the LKEKK peptide dose-dependently increased the nitric oxide (NO) production by the cells, the activity of intracellular soluble guanylate cyclase (sGC), as well as the ability of the cells for adhesion, spreading, and digestion of bacteria of the 415 virulent strain of the Salmonella typhimurium in vitro. The KKEKL peptide was simultaneously tested and proved to be inactive. Thus, the binding of CT-B and the LKEKK peptide to the receptor on the RAW 264.7 cells resulted in an increase in their NO-synthase, guanylate-cyclase and phagocytic activity.

Авторлар туралы

E. Navolotskaya

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences

Хат алмасуға жауапты Автор.
Email: navolotskaya@bibch.ru
Ресей, Pushchino, Moscow oblast, 142290

V. Sadovnikov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences

Email: navolotskaya@bibch.ru
Ресей, Pushchino, Moscow oblast, 142290

D. Zinchenko

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences

Email: navolotskaya@bibch.ru
Ресей, Pushchino, Moscow oblast, 142290

V. Vladimirov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences; Pushchino State Natural Science Institute

Email: navolotskaya@bibch.ru
Ресей, Pushchino, Moscow oblast, 142290; Pushchino, Moscow oblast, 142290

Y. Zolotarev

Institute of Molecular Genetics, Russian Academy of Sciences

Email: navolotskaya@bibch.ru
Ресей, Moscow, 123182

V. Lipkin

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Email: navolotskaya@bibch.ru
Ресей, Moscow, 117997

A. Murashev

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences

Email: navolotskaya@bibch.ru
Ресей, Pushchino, Moscow oblast, 142290

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