A New Iq-Peptide of the Kunitz Type from the Heteractis magnifica Sea Anemone Exhibits Neuroprotective Activity in a Model of Alzheimer’s Disease
- Authors: Kvetkina A.N.1, Leychenko E.V.1,2, Yurchenko E.A.1, Pislyagin E.A.1, Peigneur S.3, Tytgat Y.3, Isaeva M.P.1,2, Aminin D.L.1, Kozlovskaya E.P.1
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Affiliations:
- Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch
- School of Natural Sciences
- Catholic University of Leuven
- Issue: Vol 44, No 4 (2018)
- Pages: 416-423
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228970
- DOI: https://doi.org/10.1134/S106816201804012X
- ID: 228970
Cite item
Abstract
The HMIQ3c1 recombinant peptide (6434 Da), the Kunitz-type protease inhibitor, which belonged to the new group of the IQ-peptides from the Heteractis magnifica sea anemone was prepared. The inhibitory constant of trypsin by this peptide proved to be 5.0 × 10–8 M. rHMIQ3c1 was shown to have no interaction with eight isoforms of Kv1 channels. This peptide exhibited neuroprotective activity in a concentration of 10 μM, and the peptide increased the viability of cells of the Neuro2a murine neuroblastoma by 39.4 ± 6.6% in the presence of β-amyloid. However, the peptide did not influence the viability of the cells during their incubation with 6-hydroxydofamine.
About the authors
A. N. Kvetkina
Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch
Email: leychenko@gmail.com
Russian Federation, Vladivostok, 690022
E. V. Leychenko
Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch; School of Natural Sciences
Author for correspondence.
Email: leychenko@gmail.com
Russian Federation, Vladivostok, 690022; Vladivostok, 690090
E. A. Yurchenko
Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch
Email: leychenko@gmail.com
Russian Federation, Vladivostok, 690022
E. A. Pislyagin
Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch
Email: leychenko@gmail.com
Russian Federation, Vladivostok, 690022
S. Peigneur
Catholic University of Leuven
Email: leychenko@gmail.com
Belgium, Leuven, 3000
Y. Tytgat
Catholic University of Leuven
Email: leychenko@gmail.com
Belgium, Leuven, 3000
M. P. Isaeva
Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch; School of Natural Sciences
Email: leychenko@gmail.com
Russian Federation, Vladivostok, 690022; Vladivostok, 690090
D. L. Aminin
Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch
Email: leychenko@gmail.com
Russian Federation, Vladivostok, 690022
E. P. Kozlovskaya
Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch
Email: leychenko@gmail.com
Russian Federation, Vladivostok, 690022