Email this article The Ca2+-Regulated Photoprotein Obelin as a Target for the RNA Aptamer Selection
- Authors: Krasitskaya V.V.1,2, Davydova A.S.1, Vorobjeva M.A.1, Frank L.A.1
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Affiliations:
- Institute of Biophysics Siberian Branch, Russian Academy of Sciences
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
- Issue: Vol 44, No 3 (2018)
- Pages: 296-301
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228923
- DOI: https://doi.org/10.1134/S1068162018030093
- ID: 228923
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Abstract
A variant of the Ca2+-regulated photoprotein obelin elongated with a hexahistidine peptide from the N-terminus was developed and studied. After immobilization on a metal-affine sorbent, the hybrid protein was applied as a target for the in vitro selection of RNA aptamers. According to the data of bioluminescent solid-phase microanalysis, the selection was shown to enrich the RNA library with obelin-affine molecules.
About the authors
V. V. Krasitskaya
Institute of Biophysics Siberian Branch, Russian Academy of Sciences; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
Email: lfrank@yandex.ru
Russian Federation, Krasnoyarsk, 660036; Novosibirsk
A. S. Davydova
Institute of Biophysics Siberian Branch, Russian Academy of Sciences
Email: lfrank@yandex.ru
Russian Federation, Krasnoyarsk, 660036
M. A. Vorobjeva
Institute of Biophysics Siberian Branch, Russian Academy of Sciences
Email: lfrank@yandex.ru
Russian Federation, Krasnoyarsk, 660036
L. A. Frank
Institute of Biophysics Siberian Branch, Russian Academy of Sciences
Author for correspondence.
Email: lfrank@yandex.ru
Russian Federation, Krasnoyarsk, 660036
