电邮这篇文章 The Ca2+-Regulated Photoprotein Obelin as a Target for the RNA Aptamer Selection
- 作者: Krasitskaya V.V.1,2, Davydova A.S.1, Vorobjeva M.A.1, Frank L.A.1
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隶属关系:
- Institute of Biophysics Siberian Branch, Russian Academy of Sciences
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
- 期: 卷 44, 编号 3 (2018)
- 页面: 296-301
- 栏目: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228923
- DOI: https://doi.org/10.1134/S1068162018030093
- ID: 228923
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详细
A variant of the Ca2+-regulated photoprotein obelin elongated with a hexahistidine peptide from the N-terminus was developed and studied. After immobilization on a metal-affine sorbent, the hybrid protein was applied as a target for the in vitro selection of RNA aptamers. According to the data of bioluminescent solid-phase microanalysis, the selection was shown to enrich the RNA library with obelin-affine molecules.
作者简介
V. Krasitskaya
Institute of Biophysics Siberian Branch, Russian Academy of Sciences; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
Email: lfrank@yandex.ru
俄罗斯联邦, Krasnoyarsk, 660036; Novosibirsk
A. Davydova
Institute of Biophysics Siberian Branch, Russian Academy of Sciences
Email: lfrank@yandex.ru
俄罗斯联邦, Krasnoyarsk, 660036
M. Vorobjeva
Institute of Biophysics Siberian Branch, Russian Academy of Sciences
Email: lfrank@yandex.ru
俄罗斯联邦, Krasnoyarsk, 660036
L. Frank
Institute of Biophysics Siberian Branch, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: lfrank@yandex.ru
俄罗斯联邦, Krasnoyarsk, 660036
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