Development of Search Strategy for Peptide Inhibitors of Immune Checkpoints
- Authors: Podlesnykh S.V.1, Shanshin D.V.2, Kolosova E.A.1, Murashkin D.E.2, Shaprova O.N.2, Shcherbakov D.N.1,2, Chapoval A.I.1,3
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Affiliations:
- Russian-American Anti-Cancer Center
- ”VECTOR” State Research Center of Virology and Biotechnology
- Center for Innovations in Medicine, Biodesign Institute
- Issue: Vol 44, No 2 (2018)
- Pages: 150-157
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228851
- DOI: https://doi.org/10.1134/S1068162018020024
- ID: 228851
Cite item
Abstract
Current strategy for the blockade of molecules inhibiting T-cell immunity, the immune checkpoints (ICP), such as CTLA-4, PD-1, and B7-H1(PD-L1), using monoclonal antibodies (mAbs), showed significant clinical effects in cancer immunotherapy. In this kind of therapy, antibodies do not kill tumor cells directly, but block inhibitory signals for T lymphocytes, resulting in activation of the immune response cascade that eliminate malignant cells and lead to tumor degradation. However, the mAb preparations have some limitations, and the development of new low-molecular-weight antagonists (for example, peptides) is an important issue. In this study, we used peptide microarrays and phage display libraries to search for peptides that interact with the immune checkpoints. We found peptides that specifically bind CTLA-4, PD-1, B7-1, B7-2 and B7-H1(PD-L1) which play important role in the regulation of the immune responses. These synthetic peptides can be applied to the development of new immunomodulating drugs for cancer immunotherapy.
About the authors
S. V. Podlesnykh
Russian-American Anti-Cancer Center
Author for correspondence.
Email: step-uch@mail.ru
Russian Federation, Barnaul, Altaiskii krai, 656049
D. V. Shanshin
”VECTOR” State Research Center of Virology and Biotechnology
Email: step-uch@mail.ru
Russian Federation, Koltsovo, 630559
E. A. Kolosova
Russian-American Anti-Cancer Center
Email: step-uch@mail.ru
Russian Federation, Barnaul, Altaiskii krai, 656049
D. E. Murashkin
”VECTOR” State Research Center of Virology and Biotechnology
Email: step-uch@mail.ru
Russian Federation, Koltsovo, 630559
O. N. Shaprova
”VECTOR” State Research Center of Virology and Biotechnology
Email: step-uch@mail.ru
Russian Federation, Koltsovo, 630559
D. N. Shcherbakov
Russian-American Anti-Cancer Center; ”VECTOR” State Research Center of Virology and Biotechnology
Email: step-uch@mail.ru
Russian Federation, Barnaul, Altaiskii krai, 656049; Koltsovo, 630559
A. I. Chapoval
Russian-American Anti-Cancer Center; Center for Innovations in Medicine, Biodesign Institute
Email: step-uch@mail.ru
Russian Federation, Barnaul, Altaiskii krai, 656049; Tempe, AZ, 85287