Struggle for photostability: Bleaching mechanisms of fluorescent proteins
- 作者: Mamontova A.V.1, Grigoryev A.P.2, Tsarkova A.S.1, Lukyanov K.A.1, Bogdanov A.M.1
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隶属关系:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Faculty of Biology
- 期: 卷 43, 编号 6 (2017)
- 页面: 625-633
- 栏目: Review
- URL: https://journals.rcsi.science/1068-1620/article/view/228716
- DOI: https://doi.org/10.1134/S1068162017060085
- ID: 228716
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详细
In modern biotechnological science, there is a need for visualization of objects under study at the levels of cells, organelles, and individual molecules. Prominent among imaging methods are the methods based on the detection of fluorescence from the fluorophores with which objects under study are labeled. Fluorescent proteins (FPs) are very popular as genetically encoded fluorescent labels for lifetime imaging of target structures and processes in living systems. One of the key characteristics of FPs is their photostability, i.e., their resistance to photochemical reactions that quench the fluorescence signal. This review describes the currently known molecular mechanisms underlying photobleaching and the methods used to improve the photostability of fluorescent proteins.
作者简介
A. Mamontova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
编辑信件的主要联系方式.
Email: sphingozin@gmail.com
俄罗斯联邦, Moscow, 117997
A. Grigoryev
Faculty of Biology
Email: sphingozin@gmail.com
俄罗斯联邦, Moscow, 119234
A. Tsarkova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: sphingozin@gmail.com
俄罗斯联邦, Moscow, 117997
K. Lukyanov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: sphingozin@gmail.com
俄罗斯联邦, Moscow, 117997
A. Bogdanov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: sphingozin@gmail.com
俄罗斯联邦, Moscow, 117997
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