Identification of oncogene mutations in leukemia patients using microchip-based PCR analysis
- Authors: Bogdanov K.V.1,2, Nikulina T.S.1, Lomaia E.G.1, Slyadnev M.N.2, Zaritskey A.Y.1
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Affiliations:
- Institute of Hematology
- Lumex Group
- Issue: Vol 43, No 5 (2017)
- Pages: 544-551
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228661
- DOI: https://doi.org/10.1134/S1068162017040033
- ID: 228661
Cite item
Abstract
At present, cytogenetic and PCR-based techniques are generally used in the diagnosis of leukemia. Due to high accuracy, specificity, and sensitivity of PCR assays, they have the advantage over traditional cytogenetic tools. As a rule, the classical real time PCR is carried out in a 25-μL reaction mixture. It requires a large volume of each reagent and takes a long time to finish the test. The molecular genetic assay presented here is microchip-based real-time PCR that is optimized for simultaneous analysis of 15 oncogene mutations and one housekeeping gene abl. Moreover, this diagnostic tool requires a minimal amount of cDNA and PCR reagents (10-fold less) and a short time (2-fold less) for quantitative estimation of more than five copies of gene-target. Thus, microchip-based PCR analysis can improve the detection of oncogene mutations in leukemia patients and may be used for both early diagnostics and long-term monitoring of leukemia.
About the authors
K. V. Bogdanov
Institute of Hematology; Lumex Group
Author for correspondence.
Email: kvbogdanov@yandex.ru
Russian Federation, St. Petersburg, 197341; St. Petersburg, 192029
T. S. Nikulina
Institute of Hematology
Email: kvbogdanov@yandex.ru
Russian Federation, St. Petersburg, 197341
E. G. Lomaia
Institute of Hematology
Email: kvbogdanov@yandex.ru
Russian Federation, St. Petersburg, 197341
M. N. Slyadnev
Lumex Group
Email: kvbogdanov@yandex.ru
Russian Federation, St. Petersburg, 192029
A. Y. Zaritskey
Institute of Hematology
Email: kvbogdanov@yandex.ru
Russian Federation, St. Petersburg, 197341