Fluorophores for single-molecule localization microscopy
- Authors: Klementieva N.V.1, Bozhanova N.G.2, Zagaynova E.V.1, Lukyanov K.A.2, Mishin A.S.2
-
Affiliations:
- Nizhny Novgorod State Medical Academy
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Issue: Vol 43, No 3 (2017)
- Pages: 227-234
- Section: Review Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228505
- DOI: https://doi.org/10.1134/S1068162017030074
- ID: 228505
Cite item
Abstract
Super-resolution fluorescence microscopy allows for obtaining images with a resolution of 10–20 nm, far exceeding the diffraction limit of conventional optical microscopy (200–350 nm), and provides an opportunity to study in detail the subcellular structures and individual proteins in both living and fixed cells. Among these methods, single-molecule localization microscopy (SMLM) has become widespread. SMLM techniques are based on special fluorophores capable of photoswitching. The paper presents a classification of such fluorophores and describes their photoswitching mechanisms and successful practical applications. We discuss recent progress and prospects for the development of new effective labels suitable for SMLM.
About the authors
N. V. Klementieva
Nizhny Novgorod State Medical Academy
Author for correspondence.
Email: nvklementieva@gmail.com
Russian Federation, Nizhny Novgorod, 603005
N. G. Bozhanova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: nvklementieva@gmail.com
Russian Federation, Moscow, 117997
E. V. Zagaynova
Nizhny Novgorod State Medical Academy
Email: nvklementieva@gmail.com
Russian Federation, Nizhny Novgorod, 603005
K. A. Lukyanov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: nvklementieva@gmail.com
Russian Federation, Moscow, 117997
A. S. Mishin
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: nvklementieva@gmail.com
Russian Federation, Moscow, 117997