Polyphenolic compounds of a new biologically active extract from immortelle sandy flowers (Helichrysum arenarium (L.) Moench.)
- Authors: Grinev V.S.1, Shirokov A.A.1, Navolokin N.A.2, Polukonova N.V.2, Kurchatova M.N.2, Durnova N.A.2, Bucharskaya A.B.2, Maslyakova G.N.2
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Affiliations:
- Institute of Biochemistry and Physiology of Plants and Microorganisms
- Saratov State Medical University
- Issue: Vol 42, No 7 (2016)
- Pages: 770-776
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228340
- DOI: https://doi.org/10.1134/S1068162016070086
- ID: 228340
Cite item
Abstract
The chemical composition of a new bioactive extract from immortelle sandy (Helichrysum arenarium (L.) Moench.) was investigated. Naringin, its soluble aggregate, prunin, quercetin, apigenin, naringenin, apigenin 5-O-glucoside, and isosalipurposide were found among the flavonoids. The tested immortelle sandy extract was shown to contain known dimers, trimers, or more complex aggregates. The compounds had similar spectral but substantially different chromatographic characteristics, which can be used for their identification based on the corresponding data on flavonoids glycosylated at different positions and/or carbohydrates of various complexities, which are also characterized by similar absorption spectra and different retention times. It was found by molecular absorption spectroscopy that the immortelle extract contained 73.48 mg of flavonoids relative to rutin or 17.94 mg relative to quercetin per 1 g of dry extract weight, which corresponded to 20.99 and 5.13%, respectively. The immortelle extract obtained by the method proposed displayed antitumor activity against the transplanted sarcoma 45 and had a beneficial effect on animals in general.
About the authors
V. S. Grinev
Institute of Biochemistry and Physiology of Plants and Microorganisms
Email: nik-navolokin@yandex.ru
Russian Federation, pr. Entuziastov 13, Saratov, 410049
A. A. Shirokov
Institute of Biochemistry and Physiology of Plants and Microorganisms
Email: nik-navolokin@yandex.ru
Russian Federation, pr. Entuziastov 13, Saratov, 410049
N. A. Navolokin
Saratov State Medical University
Author for correspondence.
Email: nik-navolokin@yandex.ru
Russian Federation, ul. Bol’shaya Kazach’ya 112, Saratov, 410012
N. V. Polukonova
Saratov State Medical University
Email: nik-navolokin@yandex.ru
Russian Federation, ul. Bol’shaya Kazach’ya 112, Saratov, 410012
M. N. Kurchatova
Saratov State Medical University
Email: nik-navolokin@yandex.ru
Russian Federation, ul. Bol’shaya Kazach’ya 112, Saratov, 410012
N. A. Durnova
Saratov State Medical University
Email: nik-navolokin@yandex.ru
Russian Federation, ul. Bol’shaya Kazach’ya 112, Saratov, 410012
A. B. Bucharskaya
Saratov State Medical University
Email: nik-navolokin@yandex.ru
Russian Federation, ul. Bol’shaya Kazach’ya 112, Saratov, 410012
G. N. Maslyakova
Saratov State Medical University
Email: nik-navolokin@yandex.ru
Russian Federation, ul. Bol’shaya Kazach’ya 112, Saratov, 410012