Preparation and analysis of nuclear protein extract from Drosophila melanogaster embryos for studying transcription factors
- 作者: Shaposhnikov A.V.1, Lebedeva L.A.1, Chernioglo E.S.1, Kachaev Z.M.1, Abdrakhmanov A.1, Shidlovskii Y.V.1
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隶属关系:
- Institute of Gene Biology
- 期: 卷 42, 编号 6 (2016)
- 页面: 646-654
- 栏目: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228208
- DOI: https://doi.org/10.1134/S1068162016060108
- ID: 228208
如何引用文章
详细
Nuclear protein extract is an important substance for studying different molecular systems of the cell, particularly transcription machinery. The paper briefly describes extraction methods and provides a protocol for the preparation of extract from nuclei of Drosophila embryos using salt extraction. The extraction efficiency of transcription factors and chromatin proteins has been checked under different salt concentrations. The paper also provides data about chromatographic properties of some protein factors regulating gene activity.
作者简介
A. Shaposhnikov
Institute of Gene Biology
编辑信件的主要联系方式.
Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334
L. Lebedeva
Institute of Gene Biology
Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334
E. Chernioglo
Institute of Gene Biology
Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334
Z. Kachaev
Institute of Gene Biology
Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334
A. Abdrakhmanov
Institute of Gene Biology
Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334
Yu. Shidlovskii
Institute of Gene Biology
Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334
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