Preparation and analysis of nuclear protein extract from Drosophila melanogaster embryos for studying transcription factors


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Nuclear protein extract is an important substance for studying different molecular systems of the cell, particularly transcription machinery. The paper briefly describes extraction methods and provides a protocol for the preparation of extract from nuclei of Drosophila embryos using salt extraction. The extraction efficiency of transcription factors and chromatin proteins has been checked under different salt concentrations. The paper also provides data about chromatographic properties of some protein factors regulating gene activity.

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A. Shaposhnikov

Institute of Gene Biology

编辑信件的主要联系方式.
Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334

L. Lebedeva

Institute of Gene Biology

Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334

E. Chernioglo

Institute of Gene Biology

Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334

Z. Kachaev

Institute of Gene Biology

Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334

A. Abdrakhmanov

Institute of Gene Biology

Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334

Yu. Shidlovskii

Institute of Gene Biology

Email: shaldr23@gmail.com
俄罗斯联邦, Moscow, 119334

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