Preparation and analysis of nuclear protein extract from Drosophila melanogaster embryos for studying transcription factors
- Авторлар: Shaposhnikov A.V.1, Lebedeva L.A.1, Chernioglo E.S.1, Kachaev Z.M.1, Abdrakhmanov A.1, Shidlovskii Y.V.1
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Мекемелер:
- Institute of Gene Biology
- Шығарылым: Том 42, № 6 (2016)
- Беттер: 646-654
- Бөлім: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228208
- DOI: https://doi.org/10.1134/S1068162016060108
- ID: 228208
Дәйексөз келтіру
Аннотация
Nuclear protein extract is an important substance for studying different molecular systems of the cell, particularly transcription machinery. The paper briefly describes extraction methods and provides a protocol for the preparation of extract from nuclei of Drosophila embryos using salt extraction. The extraction efficiency of transcription factors and chromatin proteins has been checked under different salt concentrations. The paper also provides data about chromatographic properties of some protein factors regulating gene activity.
Негізгі сөздер
Авторлар туралы
A. Shaposhnikov
Institute of Gene Biology
Хат алмасуға жауапты Автор.
Email: shaldr23@gmail.com
Ресей, Moscow, 119334
L. Lebedeva
Institute of Gene Biology
Email: shaldr23@gmail.com
Ресей, Moscow, 119334
E. Chernioglo
Institute of Gene Biology
Email: shaldr23@gmail.com
Ресей, Moscow, 119334
Z. Kachaev
Institute of Gene Biology
Email: shaldr23@gmail.com
Ресей, Moscow, 119334
A. Abdrakhmanov
Institute of Gene Biology
Email: shaldr23@gmail.com
Ресей, Moscow, 119334
Yu. Shidlovskii
Institute of Gene Biology
Email: shaldr23@gmail.com
Ресей, Moscow, 119334
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