Comparison of 2D and 3D cell cultures of colorectal adenocarcinoma as models for drug screening
- Autores: Poloznikov A.1, Nikulin S.2, Bolotina L.2, Gaisina I.3, Alexeev B.1
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Afiliações:
- National Medical Research Radiological Center, Ministry of Health of the Russian Federation
- P. A. Hertsen Moscow Oncology Research Center
- Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago
- Edição: Volume 68, Nº 12 (2019)
- Páginas: 2377-2380
- Seção: Brief Communications
- URL: https://journals.rcsi.science/1066-5285/article/view/243576
- DOI: https://doi.org/10.1007/s11172-019-2716-8
- ID: 243576
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Resumo
The cytostatic effects of 5-fluorouracil, oxaliplatin, and 4-(5-methyl-5H-[1,3]dioxolo[4,5-f]-indol-7-yl)-3-(5-fluoro-1-benzofuran-3-yl)-1 H-pyrrole-2,5-dione (9-ING-41), a new glycogen synthase kinase-3 inhibitor, were evaluated in 2D and 3D models of HT-29 colorectal adenocarcinoma cells. The HT-29 cells cultured in 3D were resistant to the test drugs, while in the case of the 2D model, not only cytostatic but also cytotoxic effect was observed. The highest cytotoxic effect was detected upon the addition of 9-ING-41, at high concentrations of which the cell viability decreased to 50% after 3 h of incubation. The results indicate that 3D cultures reproduce the tumor structure and drug resistance more accurately.
Sobre autores
A. Poloznikov
National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Autor responsável pela correspondência
Email: andrey.poloznikov@nmicr.ru
Rússia, 4 ul. Koroleva, Obninsk, Kaluga Region, 249036
S. Nikulin
P. A. Hertsen Moscow Oncology Research Center
Email: andrey.poloznikov@nmicr.ru
Rússia, 3 2-i Botkinskii pr-zd, Moscow, 125284
L. Bolotina
P. A. Hertsen Moscow Oncology Research Center
Email: andrey.poloznikov@nmicr.ru
Rússia, 3 2-i Botkinskii pr-zd, Moscow, 125284
I. Gaisina
Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago
Email: andrey.poloznikov@nmicr.ru
Estados Unidos da América, 833 South Wood, Chicago, IL, 60612
B. Alexeev
National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: andrey.poloznikov@nmicr.ru
Rússia, 4 ul. Koroleva, Obninsk, Kaluga Region, 249036