Comparison of 2D and 3D cell cultures of colorectal adenocarcinoma as models for drug screening
- Authors: Poloznikov A.A.1, Nikulin S.V.2, Bolotina L.V.2, Gaisina I.N.3, Alexeev B.Y.1
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Affiliations:
- National Medical Research Radiological Center, Ministry of Health of the Russian Federation
- P. A. Hertsen Moscow Oncology Research Center
- Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago
- Issue: Vol 68, No 12 (2019)
- Pages: 2377-2380
- Section: Brief Communications
- URL: https://journals.rcsi.science/1066-5285/article/view/243576
- DOI: https://doi.org/10.1007/s11172-019-2716-8
- ID: 243576
Cite item
Abstract
The cytostatic effects of 5-fluorouracil, oxaliplatin, and 4-(5-methyl-5H-[1,3]dioxolo[4,5-f]-indol-7-yl)-3-(5-fluoro-1-benzofuran-3-yl)-1 H-pyrrole-2,5-dione (9-ING-41), a new glycogen synthase kinase-3 inhibitor, were evaluated in 2D and 3D models of HT-29 colorectal adenocarcinoma cells. The HT-29 cells cultured in 3D were resistant to the test drugs, while in the case of the 2D model, not only cytostatic but also cytotoxic effect was observed. The highest cytotoxic effect was detected upon the addition of 9-ING-41, at high concentrations of which the cell viability decreased to 50% after 3 h of incubation. The results indicate that 3D cultures reproduce the tumor structure and drug resistance more accurately.
About the authors
A. A. Poloznikov
National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Author for correspondence.
Email: andrey.poloznikov@nmicr.ru
Russian Federation, 4 ul. Koroleva, Obninsk, Kaluga Region, 249036
S. V. Nikulin
P. A. Hertsen Moscow Oncology Research Center
Email: andrey.poloznikov@nmicr.ru
Russian Federation, 3 2-i Botkinskii pr-zd, Moscow, 125284
L. V. Bolotina
P. A. Hertsen Moscow Oncology Research Center
Email: andrey.poloznikov@nmicr.ru
Russian Federation, 3 2-i Botkinskii pr-zd, Moscow, 125284
I. N. Gaisina
Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago
Email: andrey.poloznikov@nmicr.ru
United States, 833 South Wood, Chicago, IL, 60612
B. Ya. Alexeev
National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: andrey.poloznikov@nmicr.ru
Russian Federation, 4 ul. Koroleva, Obninsk, Kaluga Region, 249036