Immobilization of chymotrypsin on silver nanoparticles

Resumo

The method of immobilization of chymotrypsin based on the reduction reaction of silver nitrate with sodium borohydride in an aqueous medium in the presence of the enzyme was developed. Chymotrypsin was simultaneously a stabilizer of silver nanoparticles in solution. The dimensional characteristics, as well as proteolytic and bactericidal activity of the synthesized nanocomposites were studied. It is shown that the hybrid organic-inorganic nanocomposites based on chymotrypsin and silver nanoparticles are able to perform an increased proteolytic activity in comparison with the native enzyme in the pH range corresponding to wound surface, and suppress pathogenic flora of various microorganisms.