Immobilization of chymotrypsin on silver nanoparticles
- Authors: Pisarev O.A.1,2, Titova A.V.1, Borovikova L.N.1, Kipper A.I.1, Voroshilova T.M.3, Panarin E.F.1,2
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Affiliations:
- Institute of Macromolecular Compounds, Russian Academy of Sciences
- Peter the Great Saint-Petersburg Polytechnic University
- A. M. Nikiforov Russian Center of Emergency and Radiation Medicine
- Issue: Vol 65, No 3 (2016)
- Pages: 790-793
- Section: Full Articles
- URL: https://journals.rcsi.science/1066-5285/article/view/237648
- DOI: https://doi.org/10.1007/s11172-016-1375-2
- ID: 237648
Cite item
Abstract
The method of immobilization of chymotrypsin based on the reduction reaction of silver nitrate with sodium borohydride in an aqueous medium in the presence of the enzyme was developed. Chymotrypsin was simultaneously a stabilizer of silver nanoparticles in solution. The dimensional characteristics, as well as proteolytic and bactericidal activity of the synthesized nanocomposites were studied. It is shown that the hybrid organic-inorganic nanocomposites based on chymotrypsin and silver nanoparticles are able to perform an increased proteolytic activity in comparison with the native enzyme in the pH range corresponding to wound surface, and suppress pathogenic flora of various microorganisms.
About the authors
O. A. Pisarev
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great Saint-Petersburg Polytechnic University
Author for correspondence.
Email: chrom79@yandex.ru
Russian Federation, 31 Bolshoy prospekt, Saint-Petersburg, 199004; 29 ul. Polytechnicheskaya, Saint-Petersburg, 195251
A. V. Titova
Institute of Macromolecular Compounds, Russian Academy of Sciences
Email: chrom79@yandex.ru
Russian Federation, 31 Bolshoy prospekt, Saint-Petersburg, 199004
L. N. Borovikova
Institute of Macromolecular Compounds, Russian Academy of Sciences
Email: chrom79@yandex.ru
Russian Federation, 31 Bolshoy prospekt, Saint-Petersburg, 199004
A. I. Kipper
Institute of Macromolecular Compounds, Russian Academy of Sciences
Email: chrom79@yandex.ru
Russian Federation, 31 Bolshoy prospekt, Saint-Petersburg, 199004
T. M. Voroshilova
A. M. Nikiforov Russian Center of Emergency and Radiation Medicine
Email: chrom79@yandex.ru
Russian Federation, 54 ul. Optikov, Saint-Petersburg, 197374
E. F. Panarin
Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great Saint-Petersburg Polytechnic University
Email: chrom79@yandex.ru
Russian Federation, 31 Bolshoy prospekt, Saint-Petersburg, 199004; 29 ul. Polytechnicheskaya, Saint-Petersburg, 195251