Identification of peptides with protected functional groups by chromatography, mass spectrometry, and molecular statistics
- Authors: Milyushkin A.L.1, Laktyushina A.A.1, Buryak A.K.1
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Affiliations:
- A. N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
- Issue: Vol 66, No 1 (2017)
- Pages: 56-61
- Section: Full Articles
- URL: https://journals.rcsi.science/1066-5285/article/view/239795
- DOI: https://doi.org/10.1007/s11172-017-1699-6
- ID: 239795
Cite item
Abstract
The retention of peptides with protected functional groups on the Hypercarb carbon sorbent was studied by reversed phase HPLC. The peptides were identified by tandem mass spectrometry with electrospray ionization. The thermodynamic characteristics of adsorption (TCA) of the peptides on the graphitized thermal carbon black were determined by the molecular statistical method and compared with the retention values obtained by liquid chromatography on the Hypercarb carbon sorbent. Relationships between the calculated values of TCA and experimental retention values were shown.
About the authors
A. L. Milyushkin
A. N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Author for correspondence.
Email: lex-miles@yandex.ru
Russian Federation, Build. 4, 31 Leninsky prosp., Moscow, 119071
A. A. Laktyushina
A. N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Email: lex-miles@yandex.ru
Russian Federation, Build. 4, 31 Leninsky prosp., Moscow, 119071
A. K. Buryak
A. N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Email: lex-miles@yandex.ru
Russian Federation, Build. 4, 31 Leninsky prosp., Moscow, 119071