Identification of peptides with protected functional groups by chromatography, mass spectrometry, and molecular statistics
- 作者: Milyushkin A.1, Laktyushina A.1, Buryak A.1
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隶属关系:
- A. N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
- 期: 卷 66, 编号 1 (2017)
- 页面: 56-61
- 栏目: Full Articles
- URL: https://journals.rcsi.science/1066-5285/article/view/239795
- DOI: https://doi.org/10.1007/s11172-017-1699-6
- ID: 239795
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The retention of peptides with protected functional groups on the Hypercarb carbon sorbent was studied by reversed phase HPLC. The peptides were identified by tandem mass spectrometry with electrospray ionization. The thermodynamic characteristics of adsorption (TCA) of the peptides on the graphitized thermal carbon black were determined by the molecular statistical method and compared with the retention values obtained by liquid chromatography on the Hypercarb carbon sorbent. Relationships between the calculated values of TCA and experimental retention values were shown.
作者简介
A. Milyushkin
A. N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: lex-miles@yandex.ru
俄罗斯联邦, Build. 4, 31 Leninsky prosp., Moscow, 119071
A. Laktyushina
A. N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Email: lex-miles@yandex.ru
俄罗斯联邦, Build. 4, 31 Leninsky prosp., Moscow, 119071
A. Buryak
A. N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences
Email: lex-miles@yandex.ru
俄罗斯联邦, Build. 4, 31 Leninsky prosp., Moscow, 119071