Estimation of the degree of conjugation of oligosaccharide haptens to bovine serum albumin in the course of the squarate procedure using gel permeation HPLC

D. E. Tsvetkov; E. V. Sukhova; A. A. Karelin; Yu. E. Tsvetkov; N. E. Nifantiev

doi:10.1007/s11172-016-1680-9

Estimation of the degree of conjugation of oligosaccharide haptens to bovine serum albumin in the course of the squarate procedure using gel permeation HPLC

Authors: Tsvetkov D.E.¹, Sukhova E.V.¹, Karelin A.A.¹, Tsvetkov Y.E.¹, Nifantiev N.E.¹
Affiliations:
1. N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences
Issue: Vol 65, No 12 (2016)
Pages: 2932-2936
Section: Full Articles
URL: https://journals.rcsi.science/1066-5285/article/view/239653
DOI: https://doi.org/10.1007/s11172-016-1680-9
ID: 239653

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Abstract

An approach to estimate the degree of conjugation of oligosaccharide haptens to bovine serum albumin by the squarate procedure was proposed. The approach includes gel permeation HPLC in combination with the multichannel detection.