Crystallization and Preliminary X-ray Diffraction Study of Purine Nucleoside Phosphorylase from the Thermophilic Bacterium Thermus thermophilus Strain HB27


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
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Рұқсат жабық Тек жазылушылар үшін

Аннотация

Recombinant purine nucleoside phosphorylase from the thermophilic Thermus thermophilus strain encoded by the TT_C0194 gene was purified to homogeneity. The crystallization conditions for the enzyme were found by the vapor-diffusion technique. The crystals of the enzyme suitable for X-ray diffraction were grown under microgravity conditions by the capillary counter-diffusion method. The crystals belong to sp. gr. P212121 and have the following unit-cell parameters: a = 89.9 Å, b = 121.0 Å, c = 215.7 Å, α = β = γ = 90°. The X-ray diffraction data set suitable for the determination of the three-dimensional structure of purine nucleoside phosphorylase was collected from the grown crystals at the SPring-8 synchrotron facility to 2.5 Å resolution.

Авторлар туралы

E. Sinitsyna

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: inna@ns.crys.ras.ru
Ресей, Moscow, 117997

V. Timofeev

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,”; National Research Centre “Kurchatov Institute,”

Email: inna@ns.crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123098

N. Zhukhlistova

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,”

Email: inna@ns.crys.ras.ru
Ресей, Moscow, 119333

T. Muravieva

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: inna@ns.crys.ras.ru
Ресей, Moscow, 117997

M. Kostromina

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: inna@ns.crys.ras.ru
Ресей, Moscow, 117997

R. Esipov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Email: inna@ns.crys.ras.ru
Ресей, Moscow, 117997

I. Kuranova

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,”; National Research Centre “Kurchatov Institute,”

Хат алмасуға жауапты Автор.
Email: inna@ns.crys.ras.ru
Ресей, Moscow, 119333; Moscow, 123098

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