Crystallization and Preliminary X-ray Diffraction Study of Purine Nucleoside Phosphorylase from the Thermophilic Bacterium Thermus thermophilus Strain HB27
- Authors: Sinitsyna E.V.1, Timofeev V.I.2,3, Zhukhlistova N.E.2, Muravieva T.I.1, Kostromina M.A.1, Esipov R.S.1, Kuranova I.P.2,3
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,”
- National Research Centre “Kurchatov Institute,”
- Issue: Vol 63, No 5 (2018)
- Pages: 761-764
- Section: Structure of Macromolecular Compounds
- URL: https://journals.rcsi.science/1063-7745/article/view/192925
- DOI: https://doi.org/10.1134/S1063774518050279
- ID: 192925
Cite item
Abstract
Recombinant purine nucleoside phosphorylase from the thermophilic Thermus thermophilus strain encoded by the TT_C0194 gene was purified to homogeneity. The crystallization conditions for the enzyme were found by the vapor-diffusion technique. The crystals of the enzyme suitable for X-ray diffraction were grown under microgravity conditions by the capillary counter-diffusion method. The crystals belong to sp. gr. P212121 and have the following unit-cell parameters: a = 89.9 Å, b = 121.0 Å, c = 215.7 Å, α = β = γ = 90°. The X-ray diffraction data set suitable for the determination of the three-dimensional structure of purine nucleoside phosphorylase was collected from the grown crystals at the SPring-8 synchrotron facility to 2.5 Å resolution.
About the authors
E. V. Sinitsyna
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: inna@ns.crys.ras.ru
Russian Federation, Moscow, 117997
V. I. Timofeev
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,”; National Research Centre “Kurchatov Institute,”
Email: inna@ns.crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123098
N. E. Zhukhlistova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,”
Email: inna@ns.crys.ras.ru
Russian Federation, Moscow, 119333
T. I. Muravieva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: inna@ns.crys.ras.ru
Russian Federation, Moscow, 117997
M. A. Kostromina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: inna@ns.crys.ras.ru
Russian Federation, Moscow, 117997
R. S. Esipov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: inna@ns.crys.ras.ru
Russian Federation, Moscow, 117997
I. P. Kuranova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,”; National Research Centre “Kurchatov Institute,”
Author for correspondence.
Email: inna@ns.crys.ras.ru
Russian Federation, Moscow, 119333; Moscow, 123098