Effects of Indralin on Immunohematological Parameters and DNA Damage in Irradiated ICR (CD-1) Outbred Mice

A. Yu. Bushmanov; A. N. Osipov; M. A. Ignatov; M. D. Vorontsova; T. A. Karaulova; A. V. Gordeev; E. I. Yashkina; O. V. Nikitenko; T. M. Bychkova; N. M. Stavrakova; I. E. Andrianova; T. M. Blokhina; N. Yu. Vorobyeva; A. A. Ivanov

doi:10.1134/S1062359019110104

Effects of Indralin on Immunohematological Parameters and DNA Damage in Irradiated ICR (CD-1) Outbred Mice

Authors: Bushmanov A.Y.¹, Osipov A.N.¹^,2, Ignatov M.A.¹, Vorontsova M.D.¹, Karaulova T.A.¹, Gordeev A.V.¹, Yashkina E.I.¹, Nikitenko O.V.¹^,3, Bychkova T.M.¹^,3, Stavrakova N.M.¹, Andrianova I.E.¹, Blokhina T.M.¹^,2, Vorobyeva N.Y.¹^,2, Ivanov A.A.¹^,3^,4
Affiliations:
1. State Research Center—Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency
2. Semenov Institute of Chemical Physics, Russian Academy of Sciences
3. Federal State Budgetary Institution of Science “Russian Federation State Scientific Center—Institute of Biomedical Problems,” Russian Academy of Sciences
4. Joint Institute for Nuclear Research
Issue: Vol 46, No 11 (2019)
Pages: 1564-1570
Section: Modification of Radiation Effects
URL: https://journals.rcsi.science/1062-3590/article/view/183391
DOI: https://doi.org/10.1134/S1062359019110104
ID: 183391

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Abstract

Abstract—Intragastric administration of radioprotector indralin (B-190 drug) to outbred SPF ICR (CD-1) mice 15 minutes before X-ray irradiation at doses of 6 Gy (LD-10/30), 6.5 Gy, and 7 Gy (LD-100/30) increases the 30-day survival of the animals and positively affects the state of the central organs of immunity and hematopoiesis, as well as the number of peripheral blood leukocytes. In addition, a positive effect on the behavioral reactions of the irradiated animals and striated muscles strength was noted. In addition to these secondary features of the indralin positive effect, a significant reduction of radiation-induced DNA damage in spleenocytes was observed using flow cytometry analysis of phosphorylated histone Н2АХ (γН2АХ) (1 h and eight days after X-ray irradiation) and DNA-comet assay (eight days after X-ray irradiation). The abovementioned methods are recommended for experiments investigating the influence of radioprotective drugs on DNA damage induction and repair in the irradiated organism, as well as for initial searching of potential radioprotectors and assessment of their effectiveness level in clinical practice and experiments.