Comparative Pharmacokinetics of Nootkatone in a RAT Model of Chronic Kidney Disease VERSUS Normal Controls
- 作者: Yong-Hui Li 1, Li P.1, Tan Y.1, Cai H.1, Zhang X.1, Li Y.1, Zhang J.1
-
隶属关系:
- Hainan Provincial Key Laboratory of R&D on Tropic Plants, Hainan Medical University
- 期: 卷 74, 编号 7 (2019)
- 页面: 722-727
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183258
- DOI: https://doi.org/10.1134/S1061934819070050
- ID: 183258
如何引用文章
详细
In this study, a simple, sensitive, and rapid analytical method was developed by ultra-performance liquid chromatography (UPLC) coupled with tandem quadrupole mass spectrometry (MS) for the determination of nootkatone in normal and chronic kidney disease (CKD) rat plasma using clarithromycin as internal standard. After sample preparation by simple liquid–liquid extraction, chromatography was performed on an Acquity UPLC BEH C18 column (2.1 × 50 mm, 1.7 mm particle size) using gradient elution with the mobile phase composed of acetonitrile and water acidified with 0.1% (v/v) formic acid. Detection was achieved by electrospray ionisation MS under the multiple selective reaction monitoring mode. The linear range was 0.01‒500 ng/mL with the square regression coefficient (r) of 0.9975. The lower limit of quantification was 0.01 ng/mL. The intra- and inter-day precision was under 5% and the stability accuracy was between 3.6 and 7.0%. The average recoveries from spiked plasma samples were >83% and matrix effect was over 81%. The developed method was successfully applied to the pharmacokinetic study of nootkatone in normal and CKD rats after an oral administration of 50 mg/kg nootkaone. The results showed the cmax and area under curve of nootkaone were greatly decreased, meanwhile Vd/F and t1/2 were markedly increased in CKD rats. The pharmacokinetic characteristics of nootkatone in rats were significantly altered in CKD rats.
关键词
作者简介
Yong-Hui Li
Hainan Provincial Key Laboratory of R&D on Tropic Plants, Hainan Medical University
编辑信件的主要联系方式.
Email: lyhssl@126.com
中国, Haikou, 571199
Pei-Pei Li
Hainan Provincial Key Laboratory of R&D on Tropic Plants, Hainan Medical University
Email: 112552819@qq.com
中国, Haikou, 571199
Yin-Feng Tan
Hainan Provincial Key Laboratory of R&D on Tropic Plants, Hainan Medical University
编辑信件的主要联系方式.
Email: 112552819@qq.com
中国, Haikou, 571199
Hong-Die Cai
Hainan Provincial Key Laboratory of R&D on Tropic Plants, Hainan Medical University
Email: 112552819@qq.com
中国, Haikou, 571199
Xiao-Po Zhang
Hainan Provincial Key Laboratory of R&D on Tropic Plants, Hainan Medical University
Email: 112552819@qq.com
中国, Haikou, 571199
You-Bin Li
Hainan Provincial Key Laboratory of R&D on Tropic Plants, Hainan Medical University
Email: 112552819@qq.com
中国, Haikou, 571199
Jun-Qing Zhang
Hainan Provincial Key Laboratory of R&D on Tropic Plants, Hainan Medical University
Email: 112552819@qq.com
中国, Haikou, 571199