Determination of Methylphosphonic Acid in Human Blood Plasma by High-Performance Liquid Chromatography–Tandem Mass Spectrometry
- 作者: Baygildiev T.1, Rodin I.1, Stavrianidi A.1, Braun A.2, Shpigun O.2, Rybalchenko I.2
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隶属关系:
- Department of Chemistry
- Kostroma State Technological University
- 期: 卷 72, 编号 13 (2017)
- 页面: 1307-1311
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182714
- DOI: https://doi.org/10.1134/S1061934817130020
- ID: 182714
如何引用文章
详细
Using high-performance liquid chromatography combined with tandem mass spectrometric detection, an approach has been developed for the determination of the most stable nerve agent biomarker, methylphosphonic acid, in human blood plasma. The proposed method is based on the derivatization of methylphosphonic acid with p-bromophenacyl bromide. The optimization of conditions for human plasma sample preparation, mass spectrometric detection conditions, and gradient elution program has been performed. The proposed approach has demonstrated satisfactory reproducibility and selectivity of the determination; the limit of detection for methylphosphonic acid in human plasma was 3 ng mL–1.
作者简介
T. Baygildiev
Department of Chemistry
编辑信件的主要联系方式.
Email: timurbaychem@gmail.com
俄罗斯联邦, Moscow, 119991
I. Rodin
Department of Chemistry
Email: timurbaychem@gmail.com
俄罗斯联邦, Moscow, 119991
A. Stavrianidi
Department of Chemistry
Email: timurbaychem@gmail.com
俄罗斯联邦, Moscow, 119991
A. Braun
Kostroma State Technological University
Email: timurbaychem@gmail.com
俄罗斯联邦, Kostroma, 156005
O. Shpigun
Kostroma State Technological University
Email: timurbaychem@gmail.com
俄罗斯联邦, Kostroma, 156005
I. Rybalchenko
Kostroma State Technological University
Email: timurbaychem@gmail.com
俄罗斯联邦, Kostroma, 156005
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