Applicability of the critical chromatography concept to proteomics problems: I. Effect of the stationary phase and the size of the chromatographic column on the dependence of the retention time of peptides and proteins on the amino acid sequence
- 作者: Gorshkov A.1, Pridatchenko M.2, Perlova T.2, Tarasova I.2, Gorshkov M.2, Evreinov V.1
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隶属关系:
- Semenov Institute of Chemical Physics
- Tal’roze Institute of Energy Problems of Chemical Physics
- 期: 卷 71, 编号 1 (2016)
- 页面: 110-125
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181455
- DOI: https://doi.org/10.1134/S1061934816010056
- ID: 181455
如何引用文章
详细
A theoretical study of the effect of stationary phase parameters on the regularities of the separation of peptides and proteins using a model of critical chromatography BioLCCC and the Theoretical chromatography software created on its basis has been performed. The following problems have been discussed: mechanism of the migration of chromatographic peak along the column and its compression in gradient elution; dependence of the coefficient of protein separation on the column length; inversion of the elution order of peptides at a change of the diameter or length of the column, adsorbent nature, conditions of emergence of inversion; and anomaly of the dependence of the retention time of proteins on the adsorbent activity.
作者简介
A. Gorshkov
Semenov Institute of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
俄罗斯联邦, ul. Kosygina 4, Moscow, 119991
M. Pridatchenko
Tal’roze Institute of Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
俄罗斯联邦, Leninskii pr. 38, build. 2, Moscow, 119334
T. Perlova
Tal’roze Institute of Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
俄罗斯联邦, Leninskii pr. 38, build. 2, Moscow, 119334
I. Tarasova
Tal’roze Institute of Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
俄罗斯联邦, Leninskii pr. 38, build. 2, Moscow, 119334
M. Gorshkov
Tal’roze Institute of Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
俄罗斯联邦, Leninskii pr. 38, build. 2, Moscow, 119334
V. Evreinov
Semenov Institute of Chemical Physics
编辑信件的主要联系方式.
Email: evreinov@polymer.chph.ras.ru
俄罗斯联邦, ul. Kosygina 4, Moscow, 119991