Applicability of the critical chromatography concept to proteomics problems: I. Effect of the stationary phase and the size of the chromatographic column on the dependence of the retention time of peptides and proteins on the amino acid sequence
- Authors: Gorshkov A.V.1, Pridatchenko M.L.2, Perlova T.Y.2, Tarasova I.A.2, Gorshkov M.V.2, Evreinov V.V.1
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Affiliations:
- Semenov Institute of Chemical Physics
- Tal’roze Institute of Energy Problems of Chemical Physics
- Issue: Vol 71, No 1 (2016)
- Pages: 110-125
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181455
- DOI: https://doi.org/10.1134/S1061934816010056
- ID: 181455
Cite item
Abstract
A theoretical study of the effect of stationary phase parameters on the regularities of the separation of peptides and proteins using a model of critical chromatography BioLCCC and the Theoretical chromatography software created on its basis has been performed. The following problems have been discussed: mechanism of the migration of chromatographic peak along the column and its compression in gradient elution; dependence of the coefficient of protein separation on the column length; inversion of the elution order of peptides at a change of the diameter or length of the column, adsorbent nature, conditions of emergence of inversion; and anomaly of the dependence of the retention time of proteins on the adsorbent activity.
About the authors
A. V. Gorshkov
Semenov Institute of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Russian Federation, ul. Kosygina 4, Moscow, 119991
M. L. Pridatchenko
Tal’roze Institute of Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Leninskii pr. 38, build. 2, Moscow, 119334
T. Yu. Perlova
Tal’roze Institute of Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Leninskii pr. 38, build. 2, Moscow, 119334
I. A. Tarasova
Tal’roze Institute of Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Leninskii pr. 38, build. 2, Moscow, 119334
M. V. Gorshkov
Tal’roze Institute of Energy Problems of Chemical Physics
Email: evreinov@polymer.chph.ras.ru
Russian Federation, Leninskii pr. 38, build. 2, Moscow, 119334
V. V. Evreinov
Semenov Institute of Chemical Physics
Author for correspondence.
Email: evreinov@polymer.chph.ras.ru
Russian Federation, ul. Kosygina 4, Moscow, 119991