Development and Validation of Analytical Method for Simultaneous Estimation of Tazarotene and Hydroquinone in their Gel Formulation by Reversed-phase High Performance Liquid Chromatography
- Авторы: Yadav Rajkumar 1, Divya Y.1, Rakesh Y.1
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Учреждения:
- Department of Pharmacy, Banasthali Vidyapith
- Выпуск: Том 74, № 5 (2019)
- Страницы: 467-471
- Раздел: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183208
- DOI: https://doi.org/10.1134/S1061934819050095
- ID: 183208
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Аннотация
A simple, sensitive, rapid, precise and accurate method has been developed for simultaneous estimation of tazarotene and hydroquinone in their pharmaceutical dosage form (gel preparation). The chromatographic separation was carried out on a reversed-phase Inertsil C18 (4.6 mm I.D. × 250 mm, 5 μm) column using a mobile phase consisting of 0.02 M KH2PO4‒acetonitrile (80 : 20, v/v) at a flow rate of 1.0 mL/min and UV detection at λmax = 254 nm. The method showed linearity with the correlation coefficient of 0.999 for both tazarotene and hydroquinone over the concentration range of 5‒25 and 1‒5 µg/mL, respectively. The mean recoveries were found to be in the range of 98‒101% for both components. The method was validated as per International Conference on Harmonization guidelines for linearity, limit of detection, limit of quantification, specificity, accuracy, precision and robustness. As a result, this method can be successfully applied for routine quantification of tazarotene and hydroquinone in pharmaceutical dosage form (gel preparation).
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Об авторах
Yadav Rajkumar
Department of Pharmacy, Banasthali Vidyapith
Email: rakesh_pu@yahoo.co.in
Индия, Rajasthan, Banasthali-304022
Yadav Divya
Department of Pharmacy, Banasthali Vidyapith
Email: rakesh_pu@yahoo.co.in
Индия, Rajasthan, Banasthali-304022
Yadav Rakesh
Department of Pharmacy, Banasthali Vidyapith
Автор, ответственный за переписку.
Email: rakesh_pu@yahoo.co.in
Индия, Rajasthan, Banasthali-304022