Development and Validation of Analytical Method for Simultaneous Estimation of Tazarotene and Hydroquinone in their Gel Formulation by Reversed-phase High Performance Liquid Chromatography
- Authors: Yadav Rajkumar 1, Divya Y.1, Rakesh Y.1
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Affiliations:
- Department of Pharmacy, Banasthali Vidyapith
- Issue: Vol 74, No 5 (2019)
- Pages: 467-471
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183208
- DOI: https://doi.org/10.1134/S1061934819050095
- ID: 183208
Cite item
Abstract
A simple, sensitive, rapid, precise and accurate method has been developed for simultaneous estimation of tazarotene and hydroquinone in their pharmaceutical dosage form (gel preparation). The chromatographic separation was carried out on a reversed-phase Inertsil C18 (4.6 mm I.D. × 250 mm, 5 μm) column using a mobile phase consisting of 0.02 M KH2PO4‒acetonitrile (80 : 20, v/v) at a flow rate of 1.0 mL/min and UV detection at λmax = 254 nm. The method showed linearity with the correlation coefficient of 0.999 for both tazarotene and hydroquinone over the concentration range of 5‒25 and 1‒5 µg/mL, respectively. The mean recoveries were found to be in the range of 98‒101% for both components. The method was validated as per International Conference on Harmonization guidelines for linearity, limit of detection, limit of quantification, specificity, accuracy, precision and robustness. As a result, this method can be successfully applied for routine quantification of tazarotene and hydroquinone in pharmaceutical dosage form (gel preparation).
Keywords
About the authors
Yadav Rajkumar
Department of Pharmacy, Banasthali Vidyapith
Email: rakesh_pu@yahoo.co.in
India, Rajasthan, Banasthali-304022
Yadav Divya
Department of Pharmacy, Banasthali Vidyapith
Email: rakesh_pu@yahoo.co.in
India, Rajasthan, Banasthali-304022
Yadav Rakesh
Department of Pharmacy, Banasthali Vidyapith
Author for correspondence.
Email: rakesh_pu@yahoo.co.in
India, Rajasthan, Banasthali-304022