Identification of Regions in Apomyoglobin that Form Intermolecular Interactions in Amyloid Aggregates Using High-Performance Mass Spectrometry
- Авторы: Katina N.1, Suvorina M.1, Grigorashvili E.1, Marchenkov V.1, Ryabova N.1, Nikulin A.1, Surin A.1
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Учреждения:
- Institute of Protein Research
- Выпуск: Том 72, № 13 (2017)
- Страницы: 1271-1279
- Раздел: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182704
- DOI: https://doi.org/10.1134/S1061934817130056
- ID: 182704
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Аннотация
The formation of amyloid aggregates in human organs and tissues causes the development of incurable diseases. However, experimental studies of the mechanism of amyloid formation by proteins and the structural characteristics of amyloids are complicated because of the heterogeneity and high molecular weight of the aggregates. We used limited proteolysis and mass spectrometry for the identification of regions in the apomyoglobin polypeptide chain, which give rise to intermolecular interactions in amyloid structures. Tandem mass spectroscopy enabled the identification of regions in the myoglobin polypeptide chain, which form the core of amyloid structures. It was shown that the main structural elements for the formation of the core of amyloid fibrils in myoglobin were regions from 60 through 90 and from 97 through 124 amino acid residues. These regions coincide well with those theoretically predicted. This approach yielded important data on the structure of protein molecules in aggregates and on conformational rearrangements of apomyoglobin upon amyloid formation.
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Об авторах
N. Katina
Institute of Protein Research
Email: alan@vega.protres.ru
Россия, Pushchino, Moscow oblast, 142290
M. Suvorina
Institute of Protein Research
Email: alan@vega.protres.ru
Россия, Pushchino, Moscow oblast, 142290
E. Grigorashvili
Institute of Protein Research
Email: alan@vega.protres.ru
Россия, Pushchino, Moscow oblast, 142290
V. Marchenkov
Institute of Protein Research
Email: alan@vega.protres.ru
Россия, Pushchino, Moscow oblast, 142290
N. Ryabova
Institute of Protein Research
Email: alan@vega.protres.ru
Россия, Pushchino, Moscow oblast, 142290
A. Nikulin
Institute of Protein Research
Email: alan@vega.protres.ru
Россия, Pushchino, Moscow oblast, 142290
A. Surin
Institute of Protein Research
Автор, ответственный за переписку.
Email: alan@vega.protres.ru
Россия, Pushchino, Moscow oblast, 142290