Simultaneous determination of trimethoprim, enrofloxacin, and ciprofloxacin in blood serum of poultry
- Авторы: Kubasov A.1,2, Kochetkov P.1, Balyshev A.1,3, Lobova P.4, Abramov S.3, Abramov V.1,3
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Учреждения:
- International Research Center for the Healthcare of Humans, Animals, and Environment (MNITs OZOS)
- Institute of General and Inorganic Chemistry
- All-Russia Research Institute of Fundamental and Applied Parasitology of Animals and Plants
- All-Russia Federal Center for Quality and Standardization of Animal Medicines and Feeds
- Выпуск: Том 71, № 6 (2016)
- Страницы: 637-640
- Раздел: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181831
- DOI: https://doi.org/10.1134/S1061934816060071
- ID: 181831
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Аннотация
A method is proposed for the simultaneous determination of trimethoprim, enrofloxacin, and ciprofloxacin in blood serum of poultry using HPLC. Samples were prepared using protein precipitation. The chromatographic separation of substances was attained on a C18 column using a mixture of a 50 mM acetate buffer solution (pH 3.0) with acetonitrile (86: 14) as a mobile phase. Detection was performed at 278 nm. Linearity was observed in the concentration range 50–5000 ng/mL for trimethoprim and 10–5000 ng/mL for ciprofloxacin and enrofloxacin. Precision was ≤2.1% for enrofloxacin, ≤1.6% for ciprofloxacin, and ≤14% for trimethoprim. Accuracy was ≤10.2% for enrofloxacin, ≤9.9% for ciprofloxacin, and ≤11.9% for trimethoprim. The method was applied to pharmacokinetic studies of complex antibacterial drugs containing trimethoprim and enrofloxacin as active substances.
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Об авторах
A. Kubasov
International Research Center for the Healthcare of Humans, Animals, and Environment (MNITs OZOS); Institute of General and Inorganic Chemistry
Автор, ответственный за переписку.
Email: fobosax@mail.ru
Россия, Bol’shaya Cheremushkinskaya ul. 28, b. 11A, Moscow, 117218; Leninskii pr. 31, Moscow, 119991
P. Kochetkov
International Research Center for the Healthcare of Humans, Animals, and Environment (MNITs OZOS)
Email: fobosax@mail.ru
Россия, Bol’shaya Cheremushkinskaya ul. 28, b. 11A, Moscow, 117218
A. Balyshev
International Research Center for the Healthcare of Humans, Animals, and Environment (MNITs OZOS); All-Russia Research Institute of Fundamental and Applied Parasitology of Animals and Plants
Email: fobosax@mail.ru
Россия, Bol’shaya Cheremushkinskaya ul. 28, b. 11A, Moscow, 117218; Bol’shaya Cheremushkinskaya ul. 28, Moscow, 117218
P. Lobova
All-Russia Federal Center for Quality and Standardization of Animal Medicines and Feeds
Email: fobosax@mail.ru
Россия, Zvenigorodskoe shosse 5, Moscow, 123022
S. Abramov
All-Russia Research Institute of Fundamental and Applied Parasitology of Animals and Plants
Email: fobosax@mail.ru
Россия, Bol’shaya Cheremushkinskaya ul. 28, Moscow, 117218
V. Abramov
International Research Center for the Healthcare of Humans, Animals, and Environment (MNITs OZOS); All-Russia Research Institute of Fundamental and Applied Parasitology of Animals and Plants
Email: fobosax@mail.ru
Россия, Bol’shaya Cheremushkinskaya ul. 28, b. 11A, Moscow, 117218; Bol’shaya Cheremushkinskaya ul. 28, Moscow, 117218
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