Simultaneous determination of trimethoprim, enrofloxacin, and ciprofloxacin in blood serum of poultry
- Authors: Kubasov A.S.1,2, Kochetkov P.P.1, Balyshev A.V.1,3, Lobova P.S.4, Abramov S.V.3, Abramov V.E.1,3
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Affiliations:
- International Research Center for the Healthcare of Humans, Animals, and Environment (MNITs OZOS)
- Institute of General and Inorganic Chemistry
- All-Russia Research Institute of Fundamental and Applied Parasitology of Animals and Plants
- All-Russia Federal Center for Quality and Standardization of Animal Medicines and Feeds
- Issue: Vol 71, No 6 (2016)
- Pages: 637-640
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181831
- DOI: https://doi.org/10.1134/S1061934816060071
- ID: 181831
Cite item
Abstract
A method is proposed for the simultaneous determination of trimethoprim, enrofloxacin, and ciprofloxacin in blood serum of poultry using HPLC. Samples were prepared using protein precipitation. The chromatographic separation of substances was attained on a C18 column using a mixture of a 50 mM acetate buffer solution (pH 3.0) with acetonitrile (86: 14) as a mobile phase. Detection was performed at 278 nm. Linearity was observed in the concentration range 50–5000 ng/mL for trimethoprim and 10–5000 ng/mL for ciprofloxacin and enrofloxacin. Precision was ≤2.1% for enrofloxacin, ≤1.6% for ciprofloxacin, and ≤14% for trimethoprim. Accuracy was ≤10.2% for enrofloxacin, ≤9.9% for ciprofloxacin, and ≤11.9% for trimethoprim. The method was applied to pharmacokinetic studies of complex antibacterial drugs containing trimethoprim and enrofloxacin as active substances.
Keywords
About the authors
A. S. Kubasov
International Research Center for the Healthcare of Humans, Animals, and Environment (MNITs OZOS); Institute of General and Inorganic Chemistry
Author for correspondence.
Email: fobosax@mail.ru
Russian Federation, Bol’shaya Cheremushkinskaya ul. 28, b. 11A, Moscow, 117218; Leninskii pr. 31, Moscow, 119991
P. P. Kochetkov
International Research Center for the Healthcare of Humans, Animals, and Environment (MNITs OZOS)
Email: fobosax@mail.ru
Russian Federation, Bol’shaya Cheremushkinskaya ul. 28, b. 11A, Moscow, 117218
A. V. Balyshev
International Research Center for the Healthcare of Humans, Animals, and Environment (MNITs OZOS); All-Russia Research Institute of Fundamental and Applied Parasitology of Animals and Plants
Email: fobosax@mail.ru
Russian Federation, Bol’shaya Cheremushkinskaya ul. 28, b. 11A, Moscow, 117218; Bol’shaya Cheremushkinskaya ul. 28, Moscow, 117218
P. S. Lobova
All-Russia Federal Center for Quality and Standardization of Animal Medicines and Feeds
Email: fobosax@mail.ru
Russian Federation, Zvenigorodskoe shosse 5, Moscow, 123022
S. V. Abramov
All-Russia Research Institute of Fundamental and Applied Parasitology of Animals and Plants
Email: fobosax@mail.ru
Russian Federation, Bol’shaya Cheremushkinskaya ul. 28, Moscow, 117218
V. E. Abramov
International Research Center for the Healthcare of Humans, Animals, and Environment (MNITs OZOS); All-Russia Research Institute of Fundamental and Applied Parasitology of Animals and Plants
Email: fobosax@mail.ru
Russian Federation, Bol’shaya Cheremushkinskaya ul. 28, b. 11A, Moscow, 117218; Bol’shaya Cheremushkinskaya ul. 28, Moscow, 117218