A New Hemicyanine-based Fluorophore for Monitoring pH and Lysosome Imaging
- Autores: Yingjie Lei 1, Wu X.1, Yao Q.2
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Afiliações:
- School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology
- Sphinx Scientific Laboratory Corporation
- Edição: Volume 74, Nº 9 (2019)
- Páginas: 940-944
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183310
- DOI: https://doi.org/10.1134/S1061934819090119
- ID: 183310
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Resumo
On account of long wavelength emission and high stability, a hemi-cyanine skeleton of 2,3,3-trimethyl-1-(3-sulfopropyl)-3H-benzo[f]indolinium was condensed with 6-hydroxy-7-morpholin-4-yl-methyl-naphthalene-2-carbaldehyde in the presence of acetic anhydride. The new probe displays a sensitive absorption and fluorescence according to the pH value changes and exhibits an excellent linearity of the relative emission intensity (I670/I580) vs. pH in the pH range of 7.0–9.5 with a correlation coefficient of 0.993. Moreover, compared to the commercialized LysoTracker Green DND-26 the probe is targeting to the lysosomal imaging in HeLa cells and, thus, can be further used as a near-infrared fluorescent probe for cellular research.
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Sobre autores
Yingjie Lei
School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology
Autor responsável pela correspondência
Email: yingjlei@163.com
República Popular da China, Tianjin, 300384
Xinshi Wu
School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology
Email: yingjlei@163.com
República Popular da China, Tianjin, 300384
Qingjia Yao
Sphinx Scientific Laboratory Corporation
Email: yingjlei@163.com
República Popular da China, Tianjin, 300457