A New Hemicyanine-based Fluorophore for Monitoring pH and Lysosome Imaging
- Authors: Yingjie Lei 1, Wu X.1, Yao Q.2
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Affiliations:
- School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology
- Sphinx Scientific Laboratory Corporation
- Issue: Vol 74, No 9 (2019)
- Pages: 940-944
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183310
- DOI: https://doi.org/10.1134/S1061934819090119
- ID: 183310
Cite item
Abstract
On account of long wavelength emission and high stability, a hemi-cyanine skeleton of 2,3,3-trimethyl-1-(3-sulfopropyl)-3H-benzo[f]indolinium was condensed with 6-hydroxy-7-morpholin-4-yl-methyl-naphthalene-2-carbaldehyde in the presence of acetic anhydride. The new probe displays a sensitive absorption and fluorescence according to the pH value changes and exhibits an excellent linearity of the relative emission intensity (I670/I580) vs. pH in the pH range of 7.0–9.5 with a correlation coefficient of 0.993. Moreover, compared to the commercialized LysoTracker Green DND-26 the probe is targeting to the lysosomal imaging in HeLa cells and, thus, can be further used as a near-infrared fluorescent probe for cellular research.
Keywords
About the authors
Yingjie Lei
School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology
Author for correspondence.
Email: yingjlei@163.com
China, Tianjin, 300384
Xinshi Wu
School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology
Email: yingjlei@163.com
China, Tianjin, 300384
Qingjia Yao
Sphinx Scientific Laboratory Corporation
Email: yingjlei@163.com
China, Tianjin, 300457