Amperometric Tyrosinase Biosensors Based on Nanomaterial-Modified Electrodes for Aflatoxin M1
- Autores: Varlamova R.1, Medyantseva E.1, Khamidullina R.1, Budnikov H.1
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Afiliações:
- Butlerov Institute of Chemistry, Kazan Federal University
- Edição: Volume 74, Nº Suppl 1 (2019)
- Páginas: 59-67
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183273
- DOI: https://doi.org/10.1134/S1061934819070189
- ID: 183273
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Resumo
We propose amperometric biosensors for the determination of aflatoxin M1, based on screen-printed platinum electrodes modified by carbon nanotubes (CNT), graphene oxide (GO), gold nanoparticles (Au-NP) in chitosan, and immobilized tyrosinase. The conditions for obtaining gold nanoparticles are optimized. Aflatoxin M1 exhibits properties of a reversible tyrosinase inhibitor, which makes ensures its determination using biosensors modified by nanomaterials in the concentration range 1 × 10–11–1 × 10–6 M with LOD = 5 × 10–12 M. The kinetic studies of the enzymatic conversion of phenol in the presence of aflatoxin M1 and a tyrosinase biosensor show both two-parameter mismatch inhibition (modification with a CNT/Au-NP composite) and two-parameter coordinated inhibition (modification with GO/Au-NP). Using the proposed enzyme sensors based on tyrosinase, the procedures for determining aflatoxin M1 in dairy products are tested.
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Sobre autores
R. Varlamova
Butlerov Institute of Chemistry, Kazan Federal University
Email: emedyant@gmail.com
Rússia, Kazan, 42008
E. Medyantseva
Butlerov Institute of Chemistry, Kazan Federal University
Autor responsável pela correspondência
Email: emedyant@gmail.com
Rússia, Kazan, 42008
R. Khamidullina
Butlerov Institute of Chemistry, Kazan Federal University
Email: emedyant@gmail.com
Rússia, Kazan, 42008
H. Budnikov
Butlerov Institute of Chemistry, Kazan Federal University
Email: emedyant@gmail.com
Rússia, Kazan, 42008
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