Amperometric Tyrosinase Biosensors Based on Nanomaterial-Modified Electrodes for Aflatoxin M1
- Authors: Varlamova R.M.1, Medyantseva E.P.1, Khamidullina R.R.1, Budnikov H.C.1
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Affiliations:
- Butlerov Institute of Chemistry, Kazan Federal University
- Issue: Vol 74, No Suppl 1 (2019)
- Pages: 59-67
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183273
- DOI: https://doi.org/10.1134/S1061934819070189
- ID: 183273
Cite item
Abstract
We propose amperometric biosensors for the determination of aflatoxin M1, based on screen-printed platinum electrodes modified by carbon nanotubes (CNT), graphene oxide (GO), gold nanoparticles (Au-NP) in chitosan, and immobilized tyrosinase. The conditions for obtaining gold nanoparticles are optimized. Aflatoxin M1 exhibits properties of a reversible tyrosinase inhibitor, which makes ensures its determination using biosensors modified by nanomaterials in the concentration range 1 × 10–11–1 × 10–6 M with LOD = 5 × 10–12 M. The kinetic studies of the enzymatic conversion of phenol in the presence of aflatoxin M1 and a tyrosinase biosensor show both two-parameter mismatch inhibition (modification with a CNT/Au-NP composite) and two-parameter coordinated inhibition (modification with GO/Au-NP). Using the proposed enzyme sensors based on tyrosinase, the procedures for determining aflatoxin M1 in dairy products are tested.
About the authors
R. M. Varlamova
Butlerov Institute of Chemistry, Kazan Federal University
Email: emedyant@gmail.com
Russian Federation, Kazan, 42008
E. P. Medyantseva
Butlerov Institute of Chemistry, Kazan Federal University
Author for correspondence.
Email: emedyant@gmail.com
Russian Federation, Kazan, 42008
R. R. Khamidullina
Butlerov Institute of Chemistry, Kazan Federal University
Email: emedyant@gmail.com
Russian Federation, Kazan, 42008
H. C. Budnikov
Butlerov Institute of Chemistry, Kazan Federal University
Email: emedyant@gmail.com
Russian Federation, Kazan, 42008