Macrocyclic Antibiotics as Chiral Selectors in High-Performance Liquid Chromatography and Capillary Electrophoresis
- Autores: Shapovalova E.1, Fedorova I.1, Anan’eva I.1, Shpigun O.1
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Afiliações:
- Department of Chemistry, Moscow State University
- Edição: Volume 73, Nº 11 (2018)
- Páginas: 1064-1075
- Seção: Reviews
- URL: https://journals.rcsi.science/1061-9348/article/view/182866
- DOI: https://doi.org/10.1134/S1061934818110114
- ID: 182866
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Resumo
Data on the use of macrocyclic antibiotics (vancomycin, teicoplanin, teicoplanin aglycone, and eremomycin) for the enantioseparation of amino acids, various amino acid derivatives, α-phenylcarboxylic acids, β-blockers, and some pharmaceutical preparations in reversed-phase and polar-organic HPLC modes are summarized. It is shown that mixed chiral selectors (eremomycin–vancomycin, eremomycin–bovine serum albumin) combine the properties of two selectors. Eremomycin and macrolides (azithromycin, erythromycin, and clarithromycin) are successfully used as chiral selectors in capillary electrophoresis. Aqueous and aqueous–organic supporting electrolytes (SEs) with the addition of eremomycin or nonaqueous supporting electrolytes with the addition of a macrolide are used for enantioseparation. The use of nonaqueous supporting electrolytes decreases the adsorption of the selectors on the quartz capillary surface and enables the separation of enantiomers at a low concentration of a chiral selector. Additions of boric acid into the supporting electrolyte improve the selectivity of separation.
Sobre autores
E. Shapovalova
Department of Chemistry, Moscow State University
Email: irishan@mail.ru
Rússia, Moscow, 119991
I. Fedorova
Department of Chemistry, Moscow State University
Email: irishan@mail.ru
Rússia, Moscow, 119991
I. Anan’eva
Department of Chemistry, Moscow State University
Autor responsável pela correspondência
Email: irishan@mail.ru
Rússia, Moscow, 119991
O. Shpigun
Department of Chemistry, Moscow State University
Email: irishan@mail.ru
Rússia, Moscow, 119991