Adenosine triphosphate detection by controlled-release of carboxy fluorescein from mesoporous silica nanoparticles blocked with aptamer-based gold nanoparticles
- Autores: Ji X.1, Lv H.1, Zhang W.1, Ding C.1
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Afiliações:
- Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering
- Edição: Volume 72, Nº 4 (2017)
- Páginas: 437-444
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182408
- DOI: https://doi.org/10.1134/S1061934817040165
- ID: 182408
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Resumo
In this paper, an adenosine-5′-triphosphate (ATP) controlled-release strategy to construct a fluorescence sensing platform has been designed. In the sensing platform, because of ATP aptamer and singlestranded DNA (ssDNA)-linked mesoporous silica nanoparticles (Si-MPs) were hybridized, the pores of Si-MPs were blocked with Au nanoparticles (AuNPs) modified with ATP aptamer. Carboxy fluorescein was plugged in channels of Si-MPs. In the presence of target molecule ATP, the ATP aptamer combined with ATP and the AuNPs got away from the pore of the surface of Si-MPs modified by ssDNA. 5-Carboxyfluorescein molecule was released to allow the fluorescent detection. By monitoring the fluorescence at 518 nm, ATP could be quantitatively detected with a detection limit of 6 × 10–8 M. The linear response range was 6 × 10–8 to 1 × 10–6 M. This assay was also able to discriminate ATP from its analogs. The controlled-release aptamer-based biosensor could have an effective application in human breast cancer MCF-7 cells.
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Sobre autores
Xiaoting Ji
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering
Email: dingcaifeng2003@163.com
República Popular da China, Qingdao, 266042
Haoyuan Lv
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering
Email: dingcaifeng2003@163.com
República Popular da China, Qingdao, 266042
Wei Zhang
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering
Email: dingcaifeng2003@163.com
República Popular da China, Qingdao, 266042
Caifeng Ding
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering
Autor responsável pela correspondência
Email: dingcaifeng2003@163.com
República Popular da China, Qingdao, 266042
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