Adenosine triphosphate detection by controlled-release of carboxy fluorescein from mesoporous silica nanoparticles blocked with aptamer-based gold nanoparticles
- Authors: Ji X.1, Lv H.1, Zhang W.1, Ding C.1
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Affiliations:
- Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering
- Issue: Vol 72, No 4 (2017)
- Pages: 437-444
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182408
- DOI: https://doi.org/10.1134/S1061934817040165
- ID: 182408
Cite item
Abstract
In this paper, an adenosine-5′-triphosphate (ATP) controlled-release strategy to construct a fluorescence sensing platform has been designed. In the sensing platform, because of ATP aptamer and singlestranded DNA (ssDNA)-linked mesoporous silica nanoparticles (Si-MPs) were hybridized, the pores of Si-MPs were blocked with Au nanoparticles (AuNPs) modified with ATP aptamer. Carboxy fluorescein was plugged in channels of Si-MPs. In the presence of target molecule ATP, the ATP aptamer combined with ATP and the AuNPs got away from the pore of the surface of Si-MPs modified by ssDNA. 5-Carboxyfluorescein molecule was released to allow the fluorescent detection. By monitoring the fluorescence at 518 nm, ATP could be quantitatively detected with a detection limit of 6 × 10–8 M. The linear response range was 6 × 10–8 to 1 × 10–6 M. This assay was also able to discriminate ATP from its analogs. The controlled-release aptamer-based biosensor could have an effective application in human breast cancer MCF-7 cells.
About the authors
Xiaoting Ji
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering
Email: dingcaifeng2003@163.com
China, Qingdao, 266042
Haoyuan Lv
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering
Email: dingcaifeng2003@163.com
China, Qingdao, 266042
Wei Zhang
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering
Email: dingcaifeng2003@163.com
China, Qingdao, 266042
Caifeng Ding
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering
Author for correspondence.
Email: dingcaifeng2003@163.com
China, Qingdao, 266042