Determination of lipids and their oxidation products by IR spectrometry
- Autores: Dzhatdoeva A.1, Polimova A.1, Proskurnina E.1, Proskurnin M.2, Vladimirov Y.1
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Afiliações:
- Department of Fundamental Medicine
- Department of Chemistry
- Edição: Volume 71, Nº 6 (2016)
- Páginas: 542-548
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181746
- DOI: https://doi.org/10.1134/S1061934816060058
- ID: 181746
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Resumo
We proposed a procedure for the IR spectrometric determination of lipid hydroperoxides in biological systems. The main bands in the IR absorption spectra of linoleic acid and its hydroperoxide were identified, and analytical bands suitable for the determination of both compounds in their mixtures were selected. It was demonstrated that tert-butyl hydroperoxide can be used as an external standard for determining fatty acid hydroperoxides. Using the external standard method (calibration curve) for tert-butyl hydroperoxides, we calculated the concentration of linoleic acid hydroperoxide in its mixture with linoleic acid; it agreed with the specified values. Using the developed procedure, we estimated the concentration of hydroperoxide groups in natural cardiolipin. The results were compared to those obtained by an independent method (activated chemiluminescence).
Sobre autores
A. Dzhatdoeva
Department of Fundamental Medicine
Autor responsável pela correspondência
Email: ayshatdj@gmail.com
Rússia, Moscow, 117192
A. Polimova
Department of Fundamental Medicine
Email: ayshatdj@gmail.com
Rússia, Moscow, 117192
E. Proskurnina
Department of Fundamental Medicine
Email: ayshatdj@gmail.com
Rússia, Moscow, 117192
M. Proskurnin
Department of Chemistry
Email: ayshatdj@gmail.com
Rússia, Moscow, 119991
Yu. Vladimirov
Department of Fundamental Medicine
Email: ayshatdj@gmail.com
Rússia, Moscow, 117192