Determination of lipids and their oxidation products by IR spectrometry
- Authors: Dzhatdoeva A.A.1, Polimova A.M.1, Proskurnina E.V.1, Proskurnin M.A.2, Vladimirov Y.A.1
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Affiliations:
- Department of Fundamental Medicine
- Department of Chemistry
- Issue: Vol 71, No 6 (2016)
- Pages: 542-548
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181746
- DOI: https://doi.org/10.1134/S1061934816060058
- ID: 181746
Cite item
Abstract
We proposed a procedure for the IR spectrometric determination of lipid hydroperoxides in biological systems. The main bands in the IR absorption spectra of linoleic acid and its hydroperoxide were identified, and analytical bands suitable for the determination of both compounds in their mixtures were selected. It was demonstrated that tert-butyl hydroperoxide can be used as an external standard for determining fatty acid hydroperoxides. Using the external standard method (calibration curve) for tert-butyl hydroperoxides, we calculated the concentration of linoleic acid hydroperoxide in its mixture with linoleic acid; it agreed with the specified values. Using the developed procedure, we estimated the concentration of hydroperoxide groups in natural cardiolipin. The results were compared to those obtained by an independent method (activated chemiluminescence).
About the authors
A. A. Dzhatdoeva
Department of Fundamental Medicine
Author for correspondence.
Email: ayshatdj@gmail.com
Russian Federation, Moscow, 117192
A. M. Polimova
Department of Fundamental Medicine
Email: ayshatdj@gmail.com
Russian Federation, Moscow, 117192
E. V. Proskurnina
Department of Fundamental Medicine
Email: ayshatdj@gmail.com
Russian Federation, Moscow, 117192
M. A. Proskurnin
Department of Chemistry
Email: ayshatdj@gmail.com
Russian Federation, Moscow, 119991
Yu. A. Vladimirov
Department of Fundamental Medicine
Email: ayshatdj@gmail.com
Russian Federation, Moscow, 117192