Determination of ceftriaxone by the fluorescence quenching of quantum dots using binding with polyethyleneimine
- Autores: Karpov V.1, Spektor D.1, Beklemishev M.1
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Afiliações:
- Department of Chemistry
- Edição: Volume 71, Nº 5 (2016)
- Páginas: 519-526
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181730
- DOI: https://doi.org/10.1134/S1061934816050051
- ID: 181730
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Resumo
It is found that antibiotic ceftriaxone reacts with polyethyleneimine in aqueous solutions, probably, with the formation of a noncovalent molecular complex (complex I). The product of hydrolysis of complex I, when heated in an aqueous solution (product II), quenches the fluorescence of CdSe/CdS/ZnS coreshell- shell quantum dots, probably, because of the formation of free thiol groups. A possibility of ceftriaxone determination in aqueous solutions is demonstrated. The performance characteristics of the procedure using a spectrofluorimeter and a photocamera combined with a UV light-emitting diode are close to each other (cmin = 1 × 10–6 M, RSD = 10%). Na+, Ca2+, glucose, urea, uric acid, erythromycin, ciprofloxacin, and doxycycline produce no analytical signals under the conditions of ceftriaxone determination.
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Sobre autores
V. Karpov
Department of Chemistry
Email: mkb@analyt.chem.msu.ru
Rússia, Moscow, 119991
D. Spektor
Department of Chemistry
Email: mkb@analyt.chem.msu.ru
Rússia, Moscow, 119991
M. Beklemishev
Department of Chemistry
Autor responsável pela correspondência
Email: mkb@analyt.chem.msu.ru
Rússia, Moscow, 119991