Determination of ceftriaxone by the fluorescence quenching of quantum dots using binding with polyethyleneimine
- Authors: Karpov V.M.1, Spektor D.V.1, Beklemishev M.K.1
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Affiliations:
- Department of Chemistry
- Issue: Vol 71, No 5 (2016)
- Pages: 519-526
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181730
- DOI: https://doi.org/10.1134/S1061934816050051
- ID: 181730
Cite item
Abstract
It is found that antibiotic ceftriaxone reacts with polyethyleneimine in aqueous solutions, probably, with the formation of a noncovalent molecular complex (complex I). The product of hydrolysis of complex I, when heated in an aqueous solution (product II), quenches the fluorescence of CdSe/CdS/ZnS coreshell- shell quantum dots, probably, because of the formation of free thiol groups. A possibility of ceftriaxone determination in aqueous solutions is demonstrated. The performance characteristics of the procedure using a spectrofluorimeter and a photocamera combined with a UV light-emitting diode are close to each other (cmin = 1 × 10–6 M, RSD = 10%). Na+, Ca2+, glucose, urea, uric acid, erythromycin, ciprofloxacin, and doxycycline produce no analytical signals under the conditions of ceftriaxone determination.
About the authors
V. M. Karpov
Department of Chemistry
Email: mkb@analyt.chem.msu.ru
Russian Federation, Moscow, 119991
D. V. Spektor
Department of Chemistry
Email: mkb@analyt.chem.msu.ru
Russian Federation, Moscow, 119991
M. K. Beklemishev
Department of Chemistry
Author for correspondence.
Email: mkb@analyt.chem.msu.ru
Russian Federation, Moscow, 119991