Mineralization of TiO2 nanoparticles for the determination of titanium in rat tissues
- Autores: Devoy J.1, Brun E.2, Cosnefroy A.3, Disdier C.3, Melczer M.1, Antoine G.1, Chalansonnet M.1, Mabondzo A.3
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Afiliações:
- Department of Biomonitoring and Toxicology
- Laboratoire de Chimie Physique
- CEA, Direction des Sciences du Vivant
- Edição: Volume 71, Nº 4 (2016)
- Páginas: 418-425
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181660
- DOI: https://doi.org/10.1134/S1061934816040043
- ID: 181660
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Resumo
In order to draw appropriate conclusions about the possible adverse biological effects of titanium dioxide nanoparticles (TiO2—NPs), the so-called “dose?effect” relationship must be explored. This requires proper quantification of titanium in complex matrices such as animal organs for future toxicological studies. This study presents the method development for mineralizing TiO2—NPs for analysis of biological tissues. We compared the recovery and quantification limits of the four most commonly used mineralization methods for metal oxides. Microwave-assisted dissolution in an HNO3–HF mixture followed by H2O2 treatment produced the best results for a TiO2—NPs suspension, with 96 ± 8% recovery and a limit of quantification as low as 0.9 µg/L. This method was then used for the determination of titanium levels in tissue samples taken from rats. However, our tests revealed that even this method is not sensitive enough for quantifying titanium levels in single olfactory bulbs or hippocampus in control animals.
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Sobre autores
Jérôme Devoy
Department of Biomonitoring and Toxicology
Email: info@pleiadesonline.com
França, Vandoeuvre-les-Nancy, F-54519
Emilie Brun
Laboratoire de Chimie Physique
Email: info@pleiadesonline.com
França, Orsay, F-91140
Anne Cosnefroy
CEA, Direction des Sciences du Vivant
Email: info@pleiadesonline.com
França, Gif-sur-Yvette, F-91191
Clémence Disdier
CEA, Direction des Sciences du Vivant
Email: info@pleiadesonline.com
França, Gif-sur-Yvette, F-91191
Mathieu Melczer
Department of Biomonitoring and Toxicology
Email: info@pleiadesonline.com
França, Vandoeuvre-les-Nancy, F-54519
Guillaume Antoine
Department of Biomonitoring and Toxicology
Email: info@pleiadesonline.com
França, Vandoeuvre-les-Nancy, F-54519
Monique Chalansonnet
Department of Biomonitoring and Toxicology
Email: info@pleiadesonline.com
França, Vandoeuvre-les-Nancy, F-54519
Aloïse Mabondzo
CEA, Direction des Sciences du Vivant
Email: info@pleiadesonline.com
França, Gif-sur-Yvette, F-91191