Colorimetric Sensing of Dopamine Based on Peroxidase-Like Activity of Gold Nanoparticles
- Authors: Cuifeng Jiang 1, Pang S.1, Luo J.1, Liu X.1, Guo W.1, Jiang W.2
-
Affiliations:
- School of Materials Engineering, Yancheng Institute of Technology
- Department of Chemistry, University of Science and Technology of China (USTC)
- Issue: Vol 74, No 7 (2019)
- Pages: 679-685
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183246
- DOI: https://doi.org/10.1134/S1061934819070049
- ID: 183246
Cite item
Abstract
Development of a simple and label-free colorimetric sensing assay for dopamine is urgent, because of the important roles dopamine plays in many physiological processes. Herein, a label-free colorimetric method for determination of dopamine has been proposed based on the enhancement of the peroxidase-like activity of bare gold nanoparticles (AuNPs). The assay is based on specific interactions between protonated dopamine and gold atoms through the catechol group, which induces aggregation of AuNPs and significantly enhances their peroxidase-like activity. The catalytic activity is evaluated by chromogenic reaction of 3,3',5,5'-tetramethylbenzidine with H2O2. The mechanism for enhancement of catalytic activity is discussed. The detection limit of dopamine was 25 nM by virtue of UV-Vis spectroscopy, and 0.21 μM by naked eye. The whole detection procedure can be completed in 15 min. The proposed sensor is simple, fast, cost-effective and sensitive. These advantages make this sensor a powerful tool for the determination of dopamine in water samples.
About the authors
Cuifeng Jiang
School of Materials Engineering, Yancheng Institute of Technology
Author for correspondence.
Email: cuifengj123@163.com
China, YanchengJiangsu, 224051
Shaoping Pang
School of Materials Engineering, Yancheng Institute of Technology
Email: jiangwq@ustc.edu.cn
China, YanchengJiangsu, 224051
Juhua Luo
School of Materials Engineering, Yancheng Institute of Technology
Email: jiangwq@ustc.edu.cn
China, YanchengJiangsu, 224051
Xueran Liu
School of Materials Engineering, Yancheng Institute of Technology
Email: jiangwq@ustc.edu.cn
China, YanchengJiangsu, 224051
Wei Guo
School of Materials Engineering, Yancheng Institute of Technology
Email: jiangwq@ustc.edu.cn
China, YanchengJiangsu, 224051
Wanquan Jiang
Department of Chemistry, University of Science and Technology of China (USTC)
Author for correspondence.
Email: jiangwq@ustc.edu.cn
China, HefeiAnhui, 230026