Simultaneous Determination of Diclofenac and Esomeprazole by Reversed phase Liquid Chromatography, Dual Wavelength and Derivative Spectrophotometry
- Authors: Mokhtar M. Mabrouk 1, Hammad S.F.1, Mansour F.R.1, El-Khateeb B.Z.1
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Affiliations:
- Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University
- Issue: Vol 74, No 5 (2019)
- Pages: 458-466
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183206
- DOI: https://doi.org/10.1134/S1061934819050071
- ID: 183206
Cite item
Abstract
Three methods were developed for simultaneous determination of diclofenac and esomeprazole in their binary mixtures. Method I is dual wavelength spectrophotometry which is based on measurement of absorbance difference ΔA (281.7–315 nm) for determination of diclofenac and ∆A (262.6–301.6 nm) for determination of esomeprazole. Method II is derivative spectrophotometry which is based on measuring 1D at 301.6 nm for determination of diclofenac and 2D at 299.5 nm for determination of esomeprazole. Linearity ranges were found to be 5–25 and 2‒10 μg/mL for diclofenac and esomeprazole, respectively, using methods I and II. Method III is reversed phase HPLC method which involves separation of diclofenac and esomeprazole on XBridgeTMC18 column using a mobile phase consisting of ethanol-phosphate buffer solution (pH 4, 60 : 40, v/v). The flow rate was 1.5 mL/min with ultraviolet detection at 290 nm. The calibration graphs are linear from 2 to 120 μg/mL for diclofenac and from 5 to 60 μg/mL for esomeprazole.
About the authors
Mokhtar M. Mabrouk
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University
Email: basmaelkhateeb5@gmail.com
Egypt, El Guish Street, Tanta, 31512
Sherin F. Hammad
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University
Email: basmaelkhateeb5@gmail.com
Egypt, El Guish Street, Tanta, 31512
Fotouh R. Mansour
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University
Email: basmaelkhateeb5@gmail.com
Egypt, El Guish Street, Tanta, 31512
Basma Z. El-Khateeb
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University
Author for correspondence.
Email: basmaelkhateeb5@gmail.com
Egypt, El Guish Street, Tanta, 31512