Determination of Melatonin Hormone and Neurotransmitters in Cerebrospinal Fluid: Method Validation and Uncertainty Calculations
- Authors: Yetgin Çetin Ö.1, Karadeniz H.1, Karakaş A.1, Yenisoy-Karakaş S.1
-
Affiliations:
- Abant İzzet Baysal University, Faculty of Science and Art
- Issue: Vol 73, No 6 (2018)
- Pages: 613-625
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183077
- DOI: https://doi.org/10.1134/S1061934818060114
- ID: 183077
Cite item
Abstract
The uncertainty of measurement for well-known neurotransmitters like serotonin, noradrenalin and gamma-aminobutyric acid (GABA), glutamate and melatonin hormone with high performance liquid chromatography−fluorescence detection was calculated after the method validation. Two methods were developed for the determination of the neurotransmitters. A derivatization step was performed for the determination of GABA and glutamate. Sensitivity, method detection limit, limit of quantification, linearity, recovery, interday and intraday precision values were calculated. Low detection limit values were obtained especially for the determination of GABA and glutamate. Then, bottom-up approach was used to calculate measurement uncertainty. The critical stages of the method were evaluated. The major sources of the uncertainty budget were calibration curves, stock solution and recovery. The calculated percentage relative uncertainty values for the compounds changed between 10.1 and 16.7.
Keywords
About the authors
Özge Yetgin Çetin
Abant İzzet Baysal University, Faculty of Science and Art
Email: yenisoykarakas_s@ibu.edu.tr
Turkey, Gölköy, Bolu, 14030
Hatice Karadeniz
Abant İzzet Baysal University, Faculty of Science and Art
Email: yenisoykarakas_s@ibu.edu.tr
Turkey, Gölköy, Bolu, 14030
Alper Karakaş
Abant İzzet Baysal University, Faculty of Science and Art
Email: yenisoykarakas_s@ibu.edu.tr
Turkey, Gölköy, Bolu, 14030
Serpil Yenisoy-Karakaş
Abant İzzet Baysal University, Faculty of Science and Art
Author for correspondence.
Email: yenisoykarakas_s@ibu.edu.tr
Turkey, Gölköy, Bolu, 14030