Simultaneous Determination of Succinic Acid and Water-Soluble Vitamins by Ion-Pair High-Performance Liquid Chromatography
- Authors: Leonov K.A.1, Pustovoitov A.V.2, Vishenkova D.A.1
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Affiliations:
- Institute of Natural Resources
- OOO “Sibtest,”
- Issue: Vol 73, No 4 (2018)
- Pages: 358-363
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183000
- DOI: https://doi.org/10.1134/S1061934818040068
- ID: 183000
Cite item
Abstract
A procedure is proposed for the determination of succinic acid, riboxin, nicotinamide, and riboflavin by ion-pair HPLC with UV detection. Owing to the addition of an ion-pair modifier to the mobile phase and the selection of gradient elution conditions, the optimal retention and resolution of peaks of the components to be determined are achieved. Specificity, linearity, accuracy, and precision of the developed procedure are proved on an example of the determination of active substances in the Cerebronorm® preparation.
About the authors
K. A. Leonov
Institute of Natural Resources
Author for correspondence.
Email: leonov_k90@mail.ru
Russian Federation, Tomsk, 634034
A. V. Pustovoitov
OOO “Sibtest,”
Email: leonov_k90@mail.ru
Russian Federation, Tomsk, 634034
D. A. Vishenkova
Institute of Natural Resources
Email: leonov_k90@mail.ru
Russian Federation, Tomsk, 634034