Biochemical Detection of Cholinesterase Inhibitors in Water with a New Two-Component Chromogenic Reagent
- Authors: Pitschmann V.1, Matějovský L.2, Vetchý D.3, Staš M.2
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Affiliations:
- Oritest spol. s.r.o.
- Faculty of Environmental Technology, University of Chemistry and Technology
- Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno
- Issue: Vol 73, No 9 (2018)
- Pages: 900-905
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182828
- DOI: https://doi.org/10.1134/S1061934818090101
- ID: 182828
Cite item
Abstract
We propose a simple biochemical detector of cholinesterase inhibitors in water that is based on an enzymatic reaction. The detector was designed as a detection tube containing (i) an indication layer with an immobilized butyrylcholinesterase, (ii) a layer with substrate and chromogenic reagent and (iii) an ampoule with buffer. The chromogenic reagent contained a mixture of two triphenylmethane dyes: Guinea Green B and Fuschsin Basic. The absence of inhibitors was indicated by changing the blue color of the indication layer to violet-red. In the presence of inhibitors, the indication layer remained blue-colored. Color change was evaluated visually (by naked eye). The functionality of the detector was verified on aqueous solutions of alkaloid physostigmine and two chemical warfare agents: sarin and VX. The achieved detection limits were 0.01 μg/mL for physostigmine and sarin and 0.005 μg/mL for VX.
About the authors
V. Pitschmann
Oritest spol. s.r.o.
Email: lukas.matejovsky@vscht.cz
Czech Republic, Prague, 15000
L. Matějovský
Faculty of Environmental Technology, University of Chemistry and Technology
Author for correspondence.
Email: lukas.matejovsky@vscht.cz
Czech Republic, Prague, 16628
D. Vetchý
Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno
Email: lukas.matejovsky@vscht.cz
Czech Republic, Brno, 61242
M. Staš
Faculty of Environmental Technology, University of Chemistry and Technology
Email: lukas.matejovsky@vscht.cz
Czech Republic, Prague, 16628